Site-Specific DC Surface Signatures Influence CD4+ T Cell Co-stimulation and Lung-Homing
Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble “imprinting” signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag+ DCs in the lung and muscle-draining LNs. Higher frequencies of Ag+ CD11b+ DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag+ CD11b+ MedLN DCs were qualitatively superior at priming CD4+ T cells, which then expressed CD49a and CXCR3, and preferentially trafficked into the lung parenchyma. CD11b+ DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag+ DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4+ T cells.
CITE THIS COLLECTION
REFERENCES
- https://doi.org//10.4049/jimmunol.0990002
- https://doi.org//10.4049/jimmunol.09-90003
- https://doi.org//10.1183/16000617.00002515
- https://doi.org//10.1038/s41586-018-0499-y
- https://doi.org//10.1038/nri1869
- https://doi.org//10.1371/journal.pone.0016245
- https://doi.org//10.1164/ajrccm.164.7.2103011
- https://doi.org//10.1136/thorax.57.12.1054
- https://doi.org//10.1128/IAI.01133-10
- https://doi.org//10.1038/ni970
- https://doi.org//10.4049/jimmunol.0901903
- https://doi.org//10.1016/j.celrep.2017.08.078
- https://doi.org//10.4049/jimmunol.1401151
- https://doi.org//10.1189/jlb.1102573
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (8)
CATEGORIES
- Transplantation Immunology
- Tumour Immunology
- Immunology not elsewhere classified
- Immunology
- Veterinary Immunology
- Animal Immunology
- Genetic Immunology
- Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
- Autoimmunity
- Cellular Immunology
- Humoural Immunology and Immunochemistry
- Immunogenetics (incl. Genetic Immunology)
- Innate Immunity