Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin
D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. Biodegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. Biodegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin degradation ability through hydrolysis pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin degradation pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment.
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REFERENCES
- https://doi.org//10.1016/j.ecoenv.2010.08.036
- https://doi.org//10.1186/s12934-014-0110-7
- https://doi.org//10.1016/j.biortech.2017.12.007
- https://doi.org//10.1038/srep04475
- https://doi.org//10.1002/tox.21938
- https://doi.org//10.1016/j.talanta.2008.05.019
- https://doi.org//10.1021/acs.est.6b02253
- https://doi.org//10.1021/jf404908j
- https://doi.org//10.1038/srep08784
- https://doi.org//10.1016/j.biortech.2013.01.002
- https://doi.org//10.1007/s00253-011-3640-5
- https://doi.org//10.1016/j.jhazmat.2011.01.049
- https://doi.org//10.1016/j.biortech.2011.06.055
- https://doi.org//10.1007/s00253-011-3136-3