Presentation_1_Visualizing Oscillations in Brain Slices With Genetically Encoded Voltage Indicators.pptx (25.06 MB)
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Presentation_1_Visualizing Oscillations in Brain Slices With Genetically Encoded Voltage Indicators.pptx

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posted on 02.11.2021, 04:15 authored by Jun Kyu Rhee, Yayoi Iwamoto, Bradley J. Baker

Genetically encoded voltage indicators (GEVIs) expressed pan-neuronally were able to optically resolve bicuculline induced spontaneous oscillations in brain slices of the mouse motor cortex. Three GEVIs were used that differ in their timing of response to voltage transients as well as in their voltage ranges. The duration, number of cycles, and frequency of the recorded oscillations reflected the characteristics of each GEVI used. Multiple oscillations imaged in the same slice never originated at the same location, indicating the lack of a “hot spot” for induction of the voltage changes. Comparison of pan-neuronal, Ca2+/calmodulin-dependent protein kinase II α restricted, and parvalbumin restricted GEVI expression revealed distinct profiles for the excitatory and inhibitory cells in the spontaneous oscillations of the motor cortex. Resolving voltage fluctuations across space, time, and cell types with GEVIs represent a powerful approach to dissecting neuronal circuit activity.

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