Video_4_Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.AVI (5.08 MB)
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Video_4_Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.AVI

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posted on 24.01.2019, 04:20 authored by Wenbin Yang, Yutong Meng, Danyang Li, Quan Wen

The larval zebrafish is a promising vertebrate model organism to study neural mechanisms underlying learning and memory due to its small brain and rich behavioral repertoire. Here, we report on a high-throughput operant conditioning system for zebrafish larvae, which can simultaneously train 12 fish to associate a visual conditioned pattern with electroshocks. We find that the learning responses can be enhanced by the visual contrast, not the spatial features of the conditioned patterns, highlighted by several behavioral metrics. By further characterizing the learning curves as well as memory extinction, we demonstrate that the percentage of learners and the memory length increase as the conditioned pattern becomes darker. Finally, little difference in operant learning responses was found between AB wild-type fish and elavl3:H2B-GCaMP6f transgenic fish.

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