Video2_High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy.MP4 (2.55 MB)
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Video2_High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy.MP4

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posted on 09.05.2022, 04:47 authored by Ying Ma, Lin Ma, Juanjuan Zheng, Min Liu, Zeev Zalevsky, Peng Gao

Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system’s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.

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