Image_3_All-Optical Assay to Study Biological Neural Networks.PDF (291.98 kB)
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Image_3_All-Optical Assay to Study Biological Neural Networks.PDF

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posted on 05.07.2018, 04:10 by Wardiya Afshar Saber, Federico M. Gasparoli, Marjet G. Dirks, Frank J. Gunn-Moore, Maciej Antkowiak

We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (GECI) (jRCaMP1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.