Image_2_Differential DNA Methylation and Gene Expression Between ALV-J-Positive and ALV-J-Negative Chickens.TIF
Background: Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine against ALV-J. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases have been proven to be related to alterations in DNA methylation. In this study, we screened ALV-J-positive and -negative chickens. Subsequently, we generated and provided the genome-wide gene expression and DNA methylation profiles by MeDIP-seq and RNA-seq of ALV-J-positive and -negative chicken samples; 8,304 differentially methylated regions (DMRs) were identified by MeDIP-seq analysis (p ≤ 0.005) and 515 differentially expressed genes were identified by RNA-seq analysis (p ≤ 0.05). As a result of an integration analysis, we screened six candidate genes to identify ALV-J-negative chickens that possessed differential methylation in the promoter region. Furthermore, TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections.
History
Usage metrics
Categories
- Animal Systematics and Taxonomy
- Veterinary Anaesthesiology and Intensive Care
- Veterinary Anatomy and Physiology
- Veterinary Diagnosis and Diagnostics
- Veterinary Epidemiology
- Veterinary Immunology
- Veterinary Medicine
- Veterinary Microbiology (excl. Virology)
- Veterinary Parasitology
- Veterinary Pathology
- Veterinary Pharmacology
- Veterinary Surgery
- Veterinary Virology
- Veterinary Sciences not elsewhere classified
- Animal Physiology - Biophysics
- Animal Physiology - Cell
- Animal Physiology - Systems
- Animal Behaviour
- Animal Cell and Molecular Biology
- Animal Developmental and Reproductive Biology
- Animal Immunology
- Animal Neurobiology
- Animal Physiological Ecology
- Animal Structure and Function