Image_2_A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis).pdf
Herein, we describe a novel bunyavirus, oriental wenrivirus 1 (OWV1), discovered in moribund oriental shrimp (Penaeus chinensis) collected from a farm in China in 2016. Like most bunyaviruses, OWV1 particles were enveloped, spherical- to ovoid-shaped, and 80–115 nm in diameter. However, its genome was found to comprise four segments of (-)ssRNA. These included an L RNA segment (6,317 nt) encoding an RNA-directed RNA polymerase (RdRp) of 2,052 aa, an M RNA segment (2,978 nt) encoding a glycoprotein precursor (GPC) of 922 aa, an S1 RNA segment (1,164 nt) encoding a nucleocapsid (N) protein of 243 aa, and an S2 RNA segment (1,382 nt) encoding a putative non-structural (NSs2) protein of 401 aa. All the four OWV1 RNA segments have complementary terminal decanucleotides (5′-ACACAAAGAC and 3′-UGUGUUUCUG) identical to the genomic RNA segments of uukuviruses and similar to those of phleboviruses and tenuiviruses in the Phenuiviridae. Phylogenetic analyses revealed that the RdRp, GPC, and N proteins of OWV1 were closely related to Wēnzhōu shrimp virus 1 (WzSV-1) and Mourilyan virus (MoV) that infect black tiger shrimp (P. monodon). Phylogenetic analyses also suggested that OWV1 could be classified into a second, yet to be established, species of the Wenrivirus genus in the Phenuiviridae. These wenriviruses also clustered with Wenling crustacean virus 7 from shrimps and bunya-like brown spot virus from white-clawed crayfish. Of note there were no homologs of the NSs2 of OWV1 and MoV/WzSV-1 in GenBank, and whether other crustacean phenuiviruses also possess a similar S2 RNA segment warrants further investigation. In addition, we established a TaqMan probe-based reverse-transcription quantitative PCR method for detection of OWV1, and it was detected as 1.17 × 102—1.90 × 107 copies/ng-RNA in gills of 23 out of 32 P. chinensis samples without an obvious gross sign. However, the discovery of OWV1 highlights the expanding genomic diversity of bunyaviruses.
History
References
- https://doi.org//10.1007/s00705-019-04253-6
- https://doi.org//10.1128/IAI.3.4.617-623.1971
- https://doi.org//10.1186/1743-422X-11-89
- https://doi.org//10.1038/s41598-019-49260-4
- https://doi.org//10.1093/bioinformatics/btp348
- https://doi.org//10.1111/jfd.12826
- https://doi.org//10.1046/j.1365-2761.2003.00430.x
- https://doi.org//10.1111/jfd.13244
- https://doi.org//10.1016/j.virusres.2020.198225
- https://doi.org//10.3354/dao066091
- https://doi.org//10.1016/j.aqrep.2015.10.002
- https://doi.org//10.3390/v12030323
- https://doi.org//10.4060/cb1213t
- https://doi.org//10.1016/j.virol.2019.05.014
- https://doi.org//10.1093/sysbio/syq010
- https://doi.org//10.1073/pnas.1603827113
- https://doi.org//10.1371/journal.pone.0166320
- https://doi.org//10.1007/s00705-020-04731-2
- https://doi.org//10.7554/eLife.05378
- https://doi.org//10.1093/nar/gkl951
- https://doi.org//10.1128/JVI.79.3.1824-1835.2005
- https://doi.org//10.1093/bioinformatics/bty121
- https://doi.org//10.1099/vir.0.026724-0
- https://doi.org//10.1016/j.jviromet.2006.06.026
- https://doi.org//10.1016/j.virusres.2018.03.012
- https://doi.org//10.1016/j.aquaculture.2005.06.033
- https://doi.org//10.1038/s41586-018-0012-7
- https://doi.org//10.1038/nature20167
- https://doi.org//10.1007/s00705-019-04477-6
- https://doi.org//10.1016/j.cmi.2019.12.015
CC BY 4.0