Image_1_HSBP1 Is a Novel Interactor of FIP200 and ATG13 That Promotes Autophagy Initiation and Picornavirus Replication.tif
ATG13 and FIP200 are two subunits of the ULK kinase complex, a key regulatory component of the autophagy machinery. We have previously found that the FIP200-ATG13 subcomplex controls picornavirus replication outside its role in the ULK kinase complex and autophagy. Here, we characterized HSBP1, a very small cytoplasmic coiled-coil protein, as a novel interactor of FIP200 and ATG13 that binds these two proteins via FIP200. HSBP1 is a novel pro-picornaviral host factor since its knockdown or knockout, inhibits the replication of various picornaviruses. The anti-picornaviral function of the FIP200-ATG13 subcomplex was abolished when HSBP1 was depleted, inferring that this subcomplex negatively regulates HSBP1’s pro-picornaviral function during infections. HSBP1depletion also reduces the stability of ULK kinase complex subunits, resulting in an impairment in autophagy induction. Altogether, our data show that HSBP1 interaction with FIP200-ATG13-containing complexes is involved in the regulation of different cellular pathways.
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References
- https://doi.org//10.3791/50282
- https://doi.org//10.1111/tra.12091
- https://doi.org//10.3389/fimmu.2019.00646
- https://doi.org//10.1038/nmeth.2075
- https://doi.org//10.1016/j.immuni.2021.01.018
- https://doi.org//10.1038/s41580-018-0003-4
- https://doi.org//10.1016/j.antiviral.2018.11.011
- https://doi.org//10.1016/j.cell.2019.05.026
- https://doi.org//10.1016/j.cell.2014.11.006
- https://doi.org//10.1111/j.1462-5822.2007.01006.x
- https://doi.org//10.1016/j.semcdb.2019.08.001
- https://doi.org//10.1038/s41467-018-06674-4
- https://doi.org//10.1242/jcs.132415
- https://doi.org//10.3390/v3091549
- https://doi.org//10.1080/15548627.2020.1797280
- https://doi.org//10.1038/227680a0
- https://doi.org//10.1016/j.cmet.2019.03.003
- https://doi.org//10.1080/15548627.2021.1909407
- https://doi.org//10.1128/JVI.80.6.2705-2717.2006
- https://doi.org//10.1038/emboj.2008.42
- https://doi.org//10.1002/prot.22216
- https://doi.org//10.3390/v13071210
- https://doi.org//10.1083/jcb.201602046
- https://doi.org//10.15698/mic2017.01.550
- https://doi.org//10.1038/s41580-020-0241-0
- https://doi.org//10.1038/ncb2708
- https://doi.org//10.3390/cells6030020
- https://doi.org//10.1101/gad.12.13.1962
- https://doi.org//10.1038/nmeth.2089
- https://doi.org//10.1016/j.tcb.2013.04.010
- https://doi.org//10.1016/j.devcel.2017.11.024
- https://doi.org//10.1038/s41598-020-63397-7
- https://doi.org//10.1242/jcs.132605
- https://doi.org//10.15252/embj.201797706
- https://doi.org//10.3390/cells9122654
- https://doi.org//10.1126/sciadv.1601167
- https://doi.org//10.1099/jgv.0.000911
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