Image_1_Genome-Wide Analysis of DA1-Like Genes in Gossypium and Functional Characterization of GhDA1-1A Controlling Seed Size.TIF
Cotton (Gossypium spp.) is an economically important crop grown for natural fiber and seed oil production. DA1 is a ubiquitin receptor that determines final seed and organ size by restricting the period of cell proliferation. In the present study, we identified 7 DA1-like genes each in cultivated tetraploid (AADD) G. hirsutum and G. barbadense, and 4 and 3 DA1-like genes in their ancestral diploid G. arboreum (A2A2) and G. raimondii (D5D5), respectively. The 7 GhDA1 genes were confirmed to be distributed on four At and three Dt subgenome chromosomes in G. hirsutum. GhDA1-1A showed a high sequence similarity to AtDA1 in Arabidopsis, and they possessed the same functional domains, suggesting conserved functions. The overexpression of GhDA1-1AR301K in Arabidopsis significantly increased seed size and seed weight, indicating that GhDA1-1A is a promising target for cotton improvement. This study provides information on the molecular evolutionary properties of DA1-like genes in cotton, which will be useful for the genetic improvement of cotton.
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References
- https://doi.org//10.1002/elps.1150150171
- https://doi.org//10.1111/pbi.13249
- https://doi.org//10.1186/s12862-018-1308-9
- https://doi.org//10.1016/j.molp.2020.06.009
- https://doi.org//10.1104/pp.107.111740
- https://doi.org//10.1093/nar/gkt1223
- https://doi.org//10.1038/sj.emboj.7601362
- https://doi.org//10.1038/s41588-019-0371-5
- https://doi.org//10.1016/j.isci.2020.100948
- https://doi.org//10.1093/bioinformatics/btm404
- https://doi.org//10.1093/nar/gku949
- https://doi.org//10.3969/j.issn.1001-4942.2009.08.002
- https://doi.org//10.1016/j.pbi.2016.05.008
- https://doi.org//10.1101/gad.463608
- https://doi.org//10.1111/pbi.13298
- https://doi.org//10.1038/s41467-020-15603-3
- https://doi.org//10.1126/science.1104863
- https://doi.org//10.1038/nature11798
- https://doi.org//10.1111/jipb.12074
- https://doi.org//10.1093/molbev/mst197
- https://doi.org//10.1093/jhered/93.1.77
- https://doi.org//10.3969/j.issn.1009-0002.2013.01.028
- https://doi.org//10.1111/pbi.12696
- https://doi.org//10.3969/j.issn.1000-4440.2016.04.007
- https://doi.org//10.3969/j.issn.0517-6611.2008.23.052
- https://doi.org//10.1038/cr.2008.307
- https://doi.org//10.1105/tpc.113.115063
- https://doi.org//10.1111/pbi.12763
- https://doi.org//10.1007/s11032-015-0232-6
- https://doi.org//10.1186/s12870-015-0519-0
- https://doi.org//10.1186/s42397-019-0026-1