Image_1_Diversity of Sinorhizobium (Ensifer) meliloti Bacteriophages in the Rhizosphere of Medicago marina: Myoviruses, Filamentous and N4-Like Podovirus.pdf
Using different Sinorhizobium meliloti strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume Medicago marina. Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies showed that phages M_ort18, M_sf1.2, and M_sf3.33 belonged to the Myoviridae family with feature long, contractile tails and icosaedral head. Phages I_sf3.21 and I_sf3.10T appeared to have filamentous shape and produced turbid plaques, which is a characteristic of phages from the Inoviridae family. Phage P_ort11 is a member of the Podoviridae, with an icosahedral head and a short tail and was selected for further characterization and genome sequencing. P_ort11 contained linear, double-stranded DNA with a length of 75239 bp and 103 putative open reading frames. BLASTP analysis revealed strong similarities to Escherichia phage N4 and other N4-like phages. This is the first report of filamentous and N4-like phages that infect S. meliloti.
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References
- https://doi.org//10.1007/s00705-012-1383-y
- https://doi.org//10.1016/j.syapm.2015.07.003
- https://doi.org//10.1186/s12864-017-3729-z
- https://doi.org//10.1093/nar/gkw387
- https://doi.org//10.1007/978-1-4939-7343-9-15
- https://doi.org//10.1101/gr.6649807
- https://doi.org//10.1099/0022-1317-15-1-1
- https://doi.org//10.1111/j.1420-9101.2005.00952.x
- https://doi.org//10.1099/00221287-84-1-188
- https://doi.org//10.1128/AEM.03022-10
- https://doi.org//10.1016/j.virol.2013.11.027
- https://doi.org//10.1128/MRA.01143-18
- https://doi.org//10.1128/aem.51.5.1077-1084.1986
- https://doi.org//10.1016/S1369-5274(03)00086-9
- https://doi.org//10.1101/gad.1121403
- https://doi.org//10.1128/jb.139.1.316-317.1979
- https://doi.org//10.1046/j.1365-2958.2003.03580.x
- https://doi.org//10.1016/j.virol.2010.06.011
- https://doi.org//10.3389/fmicb.2014.00506
- https://doi.org//10.1016/j.jmb.2008.02.059
- https://doi.org//10.4161/bact.1.1.14942
- https://doi.org//10.1128/JB.00480-13
- https://doi.org//10.1128/jb.174.1.214-221.1992
- https://doi.org//10.1128/JB.01335-09
- https://doi.org//10.3390/v9070161
- https://doi.org//10.1128/aem.50.5.1171-1176.1985
- https://doi.org//10.1128/JVI.01875-14
- https://doi.org//10.1128/JVI.02684-12
- https://doi.org//10.1016/j.biortech.2010.08.046
- https://doi.org//10.1128/jb.159.1.120-124.1984
- https://doi.org//10.1128/jb.153.2.635-643.1983
- https://doi.org//10.1128/JB.187.7.2526-2631.2005
- https://doi.org//10.1146/annurev.genet.42.110807.091427
- https://doi.org//10.1006/tpbi.2002.1590
- https://doi.org//10.1073/pnas.96.5.2192
- https://doi.org//10.1007/978-1-60327-164-6
- https://doi.org//10.1016/S0065-2164(10)70007-1
- https://doi.org//10.1016/j.jsb.2017.08.005
- https://doi.org//10.1128/JVI.01353-15
- https://doi.org//10.1016/j.resmic.2014.09.006
- https://doi.org//10.1016/0038-0717(94)90174-0
- https://doi.org//10.1007/978-1-60327-164-6
- https://doi.org//10.1007/978-1-4939-7343-9_3
- https://doi.org//10.1007/978-1-60327-164-6
- https://doi.org//10.1016/j.resmic.2016.10.009
- https://doi.org//10.1016/j.resmic.2008.03.005
- https://doi.org//10.3390/biom5020647
- https://doi.org//10.1093/nar/gkw413
- https://doi.org//10.1007/s00284-009-9421-z
- https://doi.org//10.1128/jb.159.1.125-129.1984
- https://doi.org//10.1099/00221287-131-7-1779
- https://doi.org//10.1128/jb.149.1.114-122.1982
- https://doi.org//10.1073/pnas.74.5.2076
- https://doi.org//10.1186/1471-2164-14-481
- https://doi.org//10.1128/JB.01657-07
- https://doi.org//10.5423/PPJ.NT.06.2018.0100
- https://doi.org//10.1016/s0092-8674(03)00233-2
- https://doi.org//10.1038/s41598-018-26004-4
- https://doi.org//10.1016/j.micres.2013.09.011
- https://doi.org//10.1099/mic.028712-0
- https://doi.org//10.33073/pjm-2010-023
- https://doi.org//10.1016/s1161-0301(02)00076-x
- https://doi.org//10.1099/00207713-46-4-972
- https://doi.org//10.1093/oxfordjournals.molbev.a040454
- https://doi.org//10.1128/AEM.02256-13
- https://doi.org//10.1016/s0038-0717(02)00030-5
- https://doi.org//10.1007/bf00337855
- https://doi.org//10.1016/j.virol.2013.11.019
- https://doi.org//10.1016/0014-5793(85)80625-6
- https://doi.org//10.1093/molbev/msr121
- https://doi.org//10.1111/j.1365-2672.1980.tb01042.x
- https://doi.org//10.1128/jb.31.5.465-477.1936
- https://doi.org//10.1128/aem.54.1.188-196.1988
- https://doi.org//10.1007/s11104-006-9058-7
- https://doi.org//10.1128/JB.184.18.4952-4961.2002
- https://doi.org//10.1186/1743-422X-11-14
- https://doi.org//10.1007/s00705-015-2609-6
- https://doi.org//10.1099/ijs.0.02665-0
- https://doi.org//10.1371/journal.pone.0044936
- https://doi.org//10.1111/j.1462-2920.2009.01927.x
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