Image_1_Antimicrobial Susceptibility and Genomic Structure of Arcobacter skirrowii Isolates.TIF (337.34 kB)

Image_1_Antimicrobial Susceptibility and Genomic Structure of Arcobacter skirrowii Isolates.TIF

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posted on 14.12.2018 by Ingrid Hänel, Helmut Hotzel, Herbert Tomaso, Anne Busch

Campylobacter spp. are considered the most common bacterial cause of foodborne gastroenteritis in the world. The family Campylobacteraceae includes the genus Arcobacter with the three species Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii as emergent enteropathogens and potential zoonotic agents. Here, we characterized genome sequences of Arcobacter that were isolated from water poultry on farms in Germany. Isolates were cultured, identified by MALDI-TOF MS and identification was verified with PCR assays. Antibiotic susceptibility testing of isolates was carried out with erythromycin, ciprofloxacin, doxycycline, tetracycline, gentamicin, and streptomycin using the gradient strip method (E-test). We also sequenced whole genomes and predicted antibiotic resistance determinants, virulence factors, performed a phylogenetic analysis to determine the genetic relatedness of these isolates and searched for plasmids.

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