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We have assessed how varying CO₂ (180, 380, and 720 μatm) and growth light intensity (40 and 400 μmol photons m⁻² s⁻¹) affected Trichodesmium erythraeum IMS101 growth and photophysiology over free iron (Fe′) concentrations between 20 and 9,600 pM. We found significant iron dependencies of growth rate and the initial slope and maximal relative PSII electron transport rates (rP_m). Under iron-limiting concentrations, high-light increased growth rates and rP_m; possibly indicating a lower allocation of resources to iron-containing photosynthetic proteins. Higher CO₂ increased growth rates across all iron concentrations, enabled growth to occur at lower Fe′ concentrations, increased rP_m and lowered the iron half saturation constants for growth (K_m). We attribute these CO₂ responses to the operation of the CCM and the ATP spent/saved for CO₂ uptake and transport at low and high CO₂, respectively. It seems reasonable to conclude that T. erythraeum IMS101 can exhibit a high degree of phenotypic plasticity in response to CO₂, light intensity and iron-limitation. These results are important given predictions of increased dissolved CO₂ and water column stratification (i.e., higher light exposures) over the coming decades.