table_14.doc

Objective

The aim of this study was to examine the fluctuations in CD4⁺ T cells, CD8⁺ T cells, and natural CD4⁺CD25⁺FoxP3⁺T-regulatory (Treg) cells following an oral glucose tolerance test (OGTT) in participants with and those without type 2 diabetes (T2DM).

Methods

19 Japanese participants with T2DM (DM group) and 21 participants without diabetes (non-DM group) were recruited and underwent a 75-g OGTT. The cell numbers of leukocytes, lymphocytes, and the T cell compartment, such as CD4⁺, CD8⁺, and Treg, were calculated for blood samples obtained after an overnight 12 h fast and during a 75-g OGTT at 60 and 120 min.

Results

Before glucose loading, no differences in the cell numbers of leukocytes, lymphocytes, CD4⁺, CD8⁺, and Treg were observed between the DM group and the non-DM group. The proportion of CD8⁺ was significantly reduced, whereas the proportion of CD4⁺ was significantly increased, after 120 min of glucose loading in both groups. The proportion of Treg was not affected. Furthermore, a significant positive correlation was observed between the AUC_{0–120 min} of CD8⁺ and the change in the free fatty acid level following the OGTT (ρ = 0.39, P < 0.05), but not that of glucose or insulin.

Conclusion

The proportion of CD4⁺ T cells was increased and that of CD8⁺ T cells was reduced after glucose loading in both subjects with and without diabetes. These findings suggest that glucose loading dynamically affects the balance of the circulating T lymphocyte subset, regardless of glucose tolerance.