table_14.doc
The aim of this study was to examine the fluctuations in CD4+ T cells, CD8+ T cells, and natural CD4+CD25+FoxP3+T-regulatory (Treg) cells following an oral glucose tolerance test (OGTT) in participants with and those without type 2 diabetes (T2DM).
Methods19 Japanese participants with T2DM (DM group) and 21 participants without diabetes (non-DM group) were recruited and underwent a 75-g OGTT. The cell numbers of leukocytes, lymphocytes, and the T cell compartment, such as CD4+, CD8+, and Treg, were calculated for blood samples obtained after an overnight 12 h fast and during a 75-g OGTT at 60 and 120 min.
ResultsBefore glucose loading, no differences in the cell numbers of leukocytes, lymphocytes, CD4+, CD8+, and Treg were observed between the DM group and the non-DM group. The proportion of CD8+ was significantly reduced, whereas the proportion of CD4+ was significantly increased, after 120 min of glucose loading in both groups. The proportion of Treg was not affected. Furthermore, a significant positive correlation was observed between the AUC0–120 min of CD8+ and the change in the free fatty acid level following the OGTT (ρ = 0.39, P < 0.05), but not that of glucose or insulin.
ConclusionThe proportion of CD4+ T cells was increased and that of CD8+ T cells was reduced after glucose loading in both subjects with and without diabetes. These findings suggest that glucose loading dynamically affects the balance of the circulating T lymphocyte subset, regardless of glucose tolerance.
History
References
- https://doi.org//10.1111/dme.12471
- https://doi.org//10.1161/CIRCULATIONAHA.106.676379
- https://doi.org//10.1126/science.7678183
- https://doi.org//10.1172/JCI200319246
- https://doi.org//10.1038/nm.2627
- https://doi.org//10.2337/db11-1065
- https://doi.org//10.1016/j.metabol.2013.12.002
- https://doi.org//10.1111/j.2040-1124.2010.00074.x
- https://doi.org//10.1007/BF00280883
- https://doi.org//10.1053/j.gastro.2007.04.068
- https://doi.org//10.1111/j.2040-1124.2010.00041.x
- https://doi.org//10.1002/eji.201243096
- https://doi.org//10.1172/JCI114142
- https://doi.org//10.1016/0026-0495(95)90094-2
- https://doi.org//10.1016/j.diabres.2016.06.020
- https://doi.org//10.3109/s10165-007-0589-4
- https://doi.org//10.1073/pnas.1417636112
- https://doi.org//10.1016/j.trim.2009.03.001
- https://doi.org//10.1073/pnas.0903958106
- https://doi.org//10.1016/S0024-3205(01)01127-4