Table_7_The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss.xlsx (24.91 kB)
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Table_7_The Immune Atlas of Human Deciduas With Unexplained Recurrent Pregnancy Loss.xlsx

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posted on 07.06.2021, 05:59 authored by Pengfei Chen, Liying Zhou, Jiying Chen, Ying Lu, Chaoxia Cao, Shuangli Lv, Zhihong Wei, Liping Wang, Jiao Chen, Xinglin Hu, Zijing Wu, Xiaohua Zhou, Danna Su, Xuefeng Deng, Changchun Zeng, Huiyun Wang, Zuhui Pu, Ruiying Diao, Lisha Mou

Recurrent pregnancy loss (RPL) is a common fertility problem that affects 1%-2% of couples all over the world. Despite exciting discoveries regarding the important roles of the decidual natural killer cell (dNK) and regulatory T cell in pregnancy, the immune heterogeneity in patients with unexplained recurrent pregnancy loss (URPL) remains elusive. Here, we profiled the transcriptomes of 13,953 CD45+ cells from three normal and three URPL deciduas. Based on our data, the cellular composition revealed three major populations of immune cells including dNK cell, T cell, and macrophage, and four minor populations including monocytes, dendritic cell (DC), mast cell, and B cell. Especially, we identified a subpopulation of CSF1+ CD59+ KIRs-expressing dNK cells in normal deciduas, while the proportion of this subpopulation was decreased in URPL deciduas. We also identified a small subpopulation of activated dDCs that were accumulated mainly in URPL deciduas. Furthermore, our data revealed that in decidua at early pregnancy, CD8+ T cells exhibited cytotoxic properties. The decidual macrophages expressed high levels of both M1 and M2 feature genes, which made them unique to the conventional M1/M2 classification. Our single-cell data revealed the immune heterogeneity in decidua and the potentially pathogenic immune variations in URPL.

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