Table_4_Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae an.XLSX (4.04 MB)
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Table_4_Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae and Maize Silage.XLSX

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posted on 17.10.2019, 04:12 by Roland Wirth, Tamás Böjti, Gergely Lakatos, Gergely Maróti, Zoltán Bagi, Gábor Rákhely, Kornél L. Kovács

Microalgal biomass is an alternative feedstock for biogas production although its C/N ratio is usually lower than optimal, therefore co-fermentation is recommended. Biogas production from photoautotrophically grown Chlorella vulgaris (C. vulgaris) biomass (240 mL CH4 g oTS−1) and co-fermentation with maize silage (330 mL CH4 g oTS−1) has been studied in semi continuous laboratory biogas fermenters. Maize silage control yielded 310 mL CH4 g oTS−1. The microbial community and the read-based functional profiles, derived from these data, were examined during the process by using next-generation metagenome Ion Torrent sequencing technology. The read-based core microbiome consisted of 92 genera from which 60 abundant taxa were directly associated with the microbial methane producing food chain. The data-set was also analyzed in a genome-based approach. Sixty-five bins were assembled, 52 of them belonged in the core biogas producing genera identified by the read-based metagenomes. The read-based and genome-based approaches complemented and verified each other. The functional profiles indicated a variety of glycoside hydrolases. Substantial rearrangements of the methanogen functions have also been observed. Co-fermentation of algal biomass and plant biomass can be carried out for an extended period of time without process failure. The microbial members of the inoculum are well-conserved, feedstock composition changes caused mostly relative abundance alterations in the core microbiome.

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