Table_3_sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism.XLSX (317.33 kB)

Table_3_sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism.XLSX

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posted on 23.01.2020, 11:06 by Franziska Engel, Elena Ossipova, Per-Johan Jakobsson, Michael-Paul Vockenhuber, Beatrix Suess

In contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 represses pepck at the post-transcriptional level and thus modulates the intracellular level of phosphoenolpyruvate (PEP). The expression of scr5239 in turn is dependent on the global transcriptional regulator DasR, thus creating a feedback loop regulation of the central carbon metabolism. By post-transcriptional regulation of PEPCK and in all likelihood other targets, scr5239 adds an additional layer to the DasR regulatory network and provides a tool to control the metabolism dependent on the available carbon source.

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