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Table_3_Fine mapping of a novel QTL DM9.1 conferring downy mildew resistance in melon.docx (64.63 kB)

Table_3_Fine mapping of a novel QTL DM9.1 conferring downy mildew resistance in melon.docx

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posted on 2023-06-12, 04:16 authored by Xuejun Zhang, Yueming Ling, Wenli Yang, Minghua Wei, Zhenzhu Wang, Meihua Li, Yong Yang, Bin Liu, Hongping Yi, Yang-Dong Guo, Qiusheng Kong

Downy mildew (DM) is a major foliar disease globally causing great economic loss in melon production. Utilizing disease-resistant cultivars is the most efficient approach for disease control, while discovery of disease-resistant genes is crucial for the success of DM-resistant breeding. To address this problem, two F2 populations were constructed using the DM-resistant accession PI 442177 in this study, and QTLs conferring DM resistance were mapped using linkage map and QTL-seq analysis, respectively. A high-density genetic map with the length of 1096.7 cM and density of 0.7 cM was generated by using the genotyping-by-sequencing data of a F2 population. A major QTL DM9.1 with the phenotypic variance explained proportion of 24.3-37.7% was consistently detected at the early, middle, and late growth stages using the genetic map. QTL-seq analyses on the two F2 populations also validated the presence of DM9.1. Kompetitive Allele-Specific PCR (KASP) assay was further carried out to fine map DM9.1 into 1.0 Mb interval. A KASP marker co-segregating with DM9.1 was successfully developed. These results not only provided valuable information for DM-resistant gene cloning, but also offered useful markers for melon DM-resistant breeding programs.

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