Table_3_A Novel Single Cell RNA-seq Analysis of Non-Myeloid Circulating Cells in Late Sepsis.xlsx (50.42 kB)
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Table_3_A Novel Single Cell RNA-seq Analysis of Non-Myeloid Circulating Cells in Late Sepsis.xlsx

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posted on 16.08.2021, 04:47 by Dijoia B. Darden, Xiaoru Dong, Maigan A. Brusko, Lauren Kelly, Brittany Fenner, Jaimar C. Rincon, Marvin L. Dirain, Ricardo Ungaro, Dina C. Nacionales, Marie Gauthier, Michael Kladde, Todd M. Brusko, Azra Bihorac, Frederick A. Moore, Tyler Loftus, Rhonda Bacher, Lyle L. Moldawer, Alicia M. Mohr, Philip A. Efron
Background

With the successful implementation of the Surviving Sepsis Campaign guidelines, post-sepsis in-hospital mortality to sepsis continues to decrease. Those who acutely survive surgical sepsis will either rapidly recover or develop a chronic critical illness (CCI). CCI is associated with adverse long-term outcomes and 1-year mortality. Although the pathobiology of CCI remains undefined, emerging evidence suggests a post-sepsis state of pathologic myeloid activation, inducing suboptimal lymphopoiesis and erythropoiesis, as well as downstream leukocyte dysfunction. Our goal was to use single-cell RNA sequencing (scRNA-seq) to perform a detailed transcriptomic analysis of lymphoid-derived leukocytes to better understand the pathology of late sepsis.

Methods

A mixture of whole blood myeloid-enriched and Ficoll-enriched peripheral blood mononuclear cells from four late septic patients (post-sepsis day 14-21) and five healthy subjects underwent Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq).

Results

We identified unique transcriptomic patterns for multiple circulating immune cell subtypes, including B- and CD4+, CD8+, activated CD4+ and activated CD8+ T-lymphocytes, as well as natural killer (NK), NKT, and plasmacytoid dendritic cells in late sepsis patients. Analysis demonstrated that the circulating lymphoid cells maintained a transcriptome reflecting immunosuppression and low-grade inflammation. We also identified transcriptomic differences between patients with bacterial versus fungal sepsis, such as greater expression of cytotoxic genes among CD8+ T-lymphocytes in late bacterial sepsis.

Conclusion

Circulating non-myeloid cells display a unique transcriptomic pattern late after sepsis. Non-myeloid leukocytes in particular reveal a host endotype of inflammation, immunosuppression, and dysfunction, suggesting a role for precision medicine-guided immunomodulatory therapy.

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