Table_2_miR-221-3p Delivered by BMMSC-Derived Microvesicles Promotes the Development of Acute Myelocytic Leukemia.DOCX
Objective: The study aims to investigate the effects of miR-221-3p in bone marrow mesenchymal stem cell (BMMSC)-derived microvesicles (MVs) on cell cycle, proliferation and invasion of acute myelocytic leukemia (AML).
Methods: Bioinformatics was used to predict differentially expressed miRNAs (DEmiRNAs) in AML. The morphology of BMMSC-derived MVs was observed under an electron microscope, and the positional relation of MVs and OCI-AML2 cells was observed by a fluorescence microscope. MTT, Transwell, and flow cytometry assays were used to analyze the effects of MVs on OCI-AML2 cells. The targeted relationship between miR-221-3p and CDKN1C was detected by dual luciferase assay.
Results: It was verified that miR-221-3p promoted the proliferation, invasion and migration of OCI-AML2 cells, and induced the cell cycle arrest in G1/S phase as well as inhibited cell apoptosis. Further studies showed that MVs promoted the proliferation, migration and invasion of AML, and induced the cell cycle arrest in G1/S phase through miR-221-3p. It was confirmed that miR-221-3p can directly target CDKN1C to regulate cell cycle, proliferation and invasion of AML.
Conclusion: miR-221-3p in BMMSC-derived MVs regulated AML cell cycle, cell proliferation and invasion through targeting CDKN1C. miR-221-3p and CDKN1C were considered to be potential targets and biomarkers for the treatment of AML in clinic.
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References
- https://doi.org//10.1152/ajprenal.00318.2018
- https://doi.org//10.1002/prot.10018
- https://doi.org//10.1097/MOH.0000000000000439
- https://doi.org//10.1038/cdd.2014.130
- https://doi.org//10.1038/nrclinonc.2015.210
- https://doi.org//10.1182/blood-2015-07-604546
- https://doi.org//10.3324/haematol.2016.163337
- https://doi.org//10.1097/CCO.0000000000000402
- https://doi.org//10.1016/j.gene.2014.11.036
- https://doi.org//10.1016/j.canlet.2017.10.040
- https://doi.org//10.1042/BCJ20160006
- https://doi.org//10.1002/path.4306
- https://doi.org//10.1126/scisignal.aaf2797
- https://doi.org//10.1016/j.gene.2019.06.005
- https://doi.org//10.1038/nrdp.2016.10
- https://doi.org//10.1021/pr501279t
- https://doi.org//10.1007/s12072-019-09944-5
- https://doi.org//10.2174/1381612823666170413125507
- https://doi.org//10.1016/j.jconrel.2015.06.029
- https://doi.org//10.1038/srep09991
- https://doi.org//10.1016/j.bbrc.2018.02.052
- https://doi.org//10.7150/thno.33680
- https://doi.org//10.1002/ijc.30181
- https://doi.org//10.1111/jcmm.14066
- https://doi.org//10.1111/cas.13735
- https://doi.org//10.1182/blood-2002-08-2466
- https://doi.org//10.3727/096504016X14756282819385
- https://doi.org//10.3892/or.2015.4228
- https://doi.org//10.1186/s12967-015-0737-0
- https://doi.org//10.1182/blood-2015-07-604538
- https://doi.org//10.1016/j.semcancer.2017.02.006
- https://doi.org//10.1186/1748-717X-8-296
- https://doi.org//10.1007/s10620-017-4558-2
- https://doi.org//10.1038/s41419-017-0077-5
- https://doi.org//10.1007/s10456-019-09665-1
- https://doi.org//10.1080/09168451.2018.1553604
- https://doi.org//10.1016/j.biopha.2015.02.005
- https://doi.org//10.7497/j.issn.2095-3941.2013.03.001
- https://doi.org//10.1038/s41388-018-0511-x
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