Table_2_Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection.xls (40 kB)
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Table_2_Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection.xls

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posted on 22.10.2019, 04:18 by Guilherme Loss, Patricia Martins Simões, Florent Valour, Marina Farrel Cortês, Luiz Gonzaga, Marine Bergot, Sophie Trouillet-Assant, Jêrome Josse, Alan Diot, Emiliano Ricci, Ana Tereza Vasconcelos, Frédéric Laurent

Small colony variants (SCV) of Staphylococcus aureus have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with no classical auxotrophy and the emergence of rifampicin resistance. Whole Genome Sequencing (WGS) analysis showed only the loss of a 42.5 kb phage and 3 deletions, among which one targeting the rpoB gene, known to be the target of rifampicin and to be associated to SCV formation in the context of a constitutively active stringent response. Transcriptomic analysis highlighted a specific signature in the SCV strain including a complex, multi-level strategy of survival and adaptation to chronicity within the host including a protection from the inflammatory response, an evasion of the immune response, a constitutively activated stringent response and a scavenging of iron sources.

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