Table_2_Genotype-Independent Transformation and Genome Editing of Brassica napus Using a Novel Explant Material.xlsx (20.38 kB)
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Table_2_Genotype-Independent Transformation and Genome Editing of Brassica napus Using a Novel Explant Material.xlsx

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posted on 08.10.2020, 04:56 authored by Uyen Cao Chu, Sandeep Kumar, Amy Sigmund, Kari Johnson, Yinghong Li, Pamila Vongdeuane, Todd J. Jones

Agrobacterium-mediated transformation of canola (Brassica napus) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an Agrobacterium-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as CRISPR-Cas9-mediated frameshift gene knockouts.

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