Table_2_A clinical Pseudomonas juntendi strain with blaIMP−1 carried by an integrative and conjugative element in China.XLS (10.41 kB)

Table_2_A clinical Pseudomonas juntendi strain with blaIMP−1 carried by an integrative and conjugative element in China.XLS

dataset

posted on 2022-07-29, 04:47 authored by Lin Zheng, Xinfang Zhang, Lingwei Zhu, Gejin Lu, Jiayao Guan, Mingwei Liu, Jie Jing, Shiwen Sun, Ying Wang, Yang Sun, Xue Ji, Bowen Jiang, Jun Chen, Jun Liu, Ping Chen, Xuejun Guo

Objective

To precisely determine the species of a carbapenem-resistant Pseudomonas strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) 1276 formation mechanism.

Methods

Single-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE 1276 were aligned. At the same time, the transfer ability of bla_IMP−1 and the antibiotic sensitivity of Pseudomonas juntendi 18091276 were tested.

Results

This bacterium was P. juntendi, and its drug resistance mechanism is the capture of the accA4' gene cassette by the Tn402-like type 1 integron (IntI1-bla_IMP−1) to form In1886 before its capture by the ΔTn4662a-carrying ICE 1276. The acquisition of bla_IMP−1 confers carbapenem resistance to P. juntendi 18091276.

Conclusion

The formation of bla_IMP−1-carrying ICE 1276, its further integration into the chromosomes, and transposition and recombination of other elements promote bacterial gene accumulation and transmission.