Table_1_Sodium Alginate Modified Platinum Nanozymes With Highly Efficient and Robust Oxidase-Like Activity for Antioxidant Capacity and Analysis of Pr.DOCX (6.71 MB)
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Table_1_Sodium Alginate Modified Platinum Nanozymes With Highly Efficient and Robust Oxidase-Like Activity for Antioxidant Capacity and Analysis of Proanthocyanidins.DOCX

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posted on 05.08.2020, 08:20 by Shao-Bin He, Liu Yang, Xiu-Ling Lin, Hua-Ping Peng, Zhen Lin, Hao-Hua Deng, Wei Chen, Guo-Lin Hong

Platinum nanozymes exhibiting highly efficient and robust oxidase-like activity are successfully synthesized and modified using sodium alginate (SA-PtNPs). According to a steady-state dynamic assay, Michaelis-Menton constant (Km) is calculated as 11.6 μM, indicating that the affinity of SA-PtNPs toward the substrate, 3, 3′, 5, 5′-tetramethylbenzidine (TMB), is high. It shows in the paper that SA-PtNPs exhibit a significant oxidant effect on substrate-O2 to produce O2•- as an oxidase mimic. Moreover, the oxidase-like activity fluctuated slightly under changes in environmental pH and incubation time, implying that SA can increase the dispersibility and stability of PtNPs. A colorimetric assay for oligomeric proanthocyanidins (OPC) was realized given how few explorations of the former there are. We found that the significant inhibitory effect of OPC on the oxidase-like activity is due to the competitive effect between OPC and TMB for binding to the active site of SA-PtNPs, resulting in a color change. Under optimal conditions, the logarithmic value of the chromogenic difference (ΔA450nm) to OPC concentration was linear (4–32.5 μM, r = 0.999) with a limit of detection (LOD) of 2.0 μM. The antioxidant capacity of OPC obtained by the Soxhlet extraction method from grape seeds was 2.85 U/mg. The recovery from the experiment in which OPC was added to grape seeds ranged from 97.0 to 98.6% (RSDs of 0.5–3.4%), suggesting a high accuracy in OPC detection. These findings are important because OPC is an internationally recognized antioxidant that eliminates free radicals in the human body and, therefore, may prevent a variety of diseases. Thus, we envisage that this Pt nanozyme-based assay may be prevalent for antioxidant capacity evaluation and analytical applications.

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