Table_1_Phage vB_PaeS-PAJD-1 Rescues Murine Mastitis Infected With Multidrug-Resistant Pseudomonas aeruginosa.docx
Pseudomonas aeruginosa is a Gram-negative pathogen that causes a variety of infections in humans and animals. Due to the inappropriate use of antibiotics, multi-drug resistant (MDR) P. aeruginosa strains have emerged and are prevailing. In recent years, cow mastitis caused by MDR P. aeruginosa has attracted attention. In this study, a microbial community analysis revealed that P. aeruginosa could be a cause of pathogen-induced cow mastitis. Five MDR P. aeruginosa strains were isolated from milk diagnosed as mastitis positive. To seek an alternative antibacterial agent against MDR, P. aeruginosa, a lytic phage, designated vB_PaeS_PAJD-1 (PAJD-1), was isolated from dairy farm sewage. PAJD-1 was morphologically classified as Siphoviridae and was estimated to be about 57.9 kb. Phage PAJD-1 showed broad host ranges and a strong lytic ability. A one-step growth curve analysis showed a relatively short latency period (20 min) and a relatively high burst size (223 PFU per infected cell). Phage PAJD-1 remained stable over wide temperature and pH ranges. Intramammary-administered PAJD-1 reduced bacterial concentrations and repaired mammary glands in mice with mastitis induced by MDR P. aeruginosa. Furthermore, the cell wall hydrolase (termed endolysin) from phage PAJD-1 exhibited a strong bacteriolytic and a wide antibacterial spectrum against MDR P. aeruginosa. These findings present phage PAJD-1 as a candidate for phagotherapy against MDR P. aeruginosa infection.
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References
- https://doi.org//10.1093/nar/25.17.3389
- https://doi.org//10.1186/s12864-017-3729-z
- https://doi.org//10.1016/j.cels.2015.08.013
- https://doi.org//10.1038/s41467-020-14363-4
- https://doi.org//10.1371/journal.pone.0105680
- https://doi.org//10.1016/B978-0-12-394598-3.00006-X
- https://doi.org//10.1371/journal.pone.0184218
- https://doi.org//10.1038/nmeth.f.303
- https://doi.org//10.1038/ismej.2012.8
- https://doi.org//10.1186/s12941-020-00389-5
- https://doi.org//10.1016/j.bioorg.2019.103121
- https://doi.org//10.1128/AEM.03006-05
- https://doi.org//10.1093/bioinformatics/btq461
- https://doi.org//10.1016/j.vetmic.2020.108644
- https://doi.org//10.22038/ijbms.2020.47826.11004
- https://doi.org//10.1371/journal.pone.0047742
- https://doi.org//10.1111/j.1462-5822.2007.00999.x
- https://doi.org//10.1016/j.copbio.2020.08.014
- https://doi.org//10.1146/annurev-micro-090817-062535
- https://doi.org//10.1186/s13006-015-0038-5
- https://doi.org//10.1016/S1473-3099(18)30482-1
- https://doi.org//10.3389/fmicb.2020.619542
- https://doi.org//10.1128/AEM.02900-18
- https://doi.org//10.1111/asj.12815
- https://doi.org//10.3168/jds.2018-15326
- https://doi.org//10.3389/fmicb.2020.599906
- https://doi.org//10.1111/tbed.12704
- https://doi.org//10.3389/fmicb.2020.00998
- https://doi.org//10.1111/mec.14542
- https://doi.org//10.1017/S0022029914000454
- https://doi.org//10.3389/fmicb.2020.593988
- https://doi.org//10.3389/fmicb.2016.00208
- https://doi.org//10.1016/j.jbiosc.2020.02.001
- https://doi.org//10.1111/1574-6976.12088
- https://doi.org//10.1128/JB.182.18.5114-5120.2000
- https://doi.org//10.3389/fphar.2019.00513
- https://doi.org//10.1128/AAC.00024-19
- https://doi.org//10.1016/j.micres.2016.04.008
- https://doi.org//10.3390/ani11020279
- https://doi.org//10.1186/gb-2011-12-6-r60
- https://doi.org//10.12688/f1000research.19509.1
- https://doi.org//10.1007/s12275-017-6431-6
- https://doi.org//10.1128/AEM.00380-18
- https://doi.org//10.3389/fmicb.2016.00934
- https://doi.org//10.1111/j.1749-4486.2009.01973.x
- https://doi.org//10.3389/fmicb.2018.01778
- https://doi.org//10.1007/s10482-017-0912-9
- https://doi.org//10.1016/j.cell.2019.09.015
- https://doi.org//10.1093/femsle/fnw186