Table_1_Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.XLSX (12.03 kB)

Table_1_Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.XLSX

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posted on 14.02.2020 by Thomas K. Lavin, Lei Jin, Nicholas E. Lea, Ian R. Wickersham

Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent “helper” adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (ΔG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.

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