Table_1_Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, “Gao-ben”) by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure.DOCX
Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as “Gao-ben,” refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as “Gao-ben” in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The “Gao-ben” is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true “Gao-ben” and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine.
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References
- https://doi.org//10.1371/journal.pone.0008613
- https://doi.org//10.1073/pnas.1104551108
- https://doi.org//10.1055/s-2007-990227
- https://doi.org//10.1016/S0168-9525(03)00118-5
- https://doi.org//10.1093/nar/gkm233
- https://doi.org//10.1016/j.tig.2015.01.002
- https://doi.org//10.1007/PL00006217
- https://doi.org//10.1006/jmbi.2001.4807
- https://doi.org//10.1046/j.1365-294X.2002.01485.x
- https://doi.org//10.1186/1471-2148-7-90
- https://doi.org//10.1371/journal.pone.0067818
- https://doi.org//10.1098/rspb.2002.2218
- https://doi.org//10.1093/nar/24.15.2857
- https://doi.org//10.1186/1745-6150-5-4
- https://doi.org//10.1016/j.gene.2008.10.012
- https://doi.org//10.1006/mpev.1995.1028
- https://doi.org//10.1016/j.ympev.2012.01.026
- https://doi.org//10.1093/molbev/msw054
- https://doi.org//10.1093/nar/27.15.3071
- https://doi.org//10.1017/S1355838201002515
- https://doi.org//10.1007/PL00006143
- https://doi.org//10.1111/pbi.12419
- https://doi.org//10.1261/rna.617107
- https://doi.org//10.1186/1741-7015-11-222
- https://doi.org//10.1261/rna.7204505
- https://doi.org//10.1186/1756-0500-1-91
- https://doi.org//10.1186/1471-2105-7-498
- https://doi.org//10.1093/molbev/msi099
- https://doi.org//10.1055/s-2006-951695
- https://doi.org//10.1038/s41598-017-09769-y
- https://doi.org//10.1371/journal.pone.0131185
- https://doi.org//10.3389/fpls.2015.00840
- https://doi.org//10.1016/j.ympev.2009.05.029
- https://doi.org//10.4236/ajps.2018.96084
- https://doi.org//10.1080/00087114.2008.10589644
- https://doi.org//10.1038/s41598-017-09769-y
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