Frontiers
Browse

Table_1_Hatchery tanks induce intense reduction in microbiota diversity associated with gills and guts of two endemic species of the São Francisco River.XLSX

Download (571.54 kB)
dataset
posted on 2022-12-02, 04:44 authored by Maria Rosilene Alves Damasceno, Camila Gracyelle de Carvalho Lemes, Lucélia Sandra Silva Barbosa Braga, Polyana Cristine Tizioto, Horácio Montenegro, Marcela Paduan, Josielda Gomes Pereira, Isabella Ferreira Cordeiro, Lorrana Cachuite Mendes Rocha, Sibele Aryadne da Silva, Angélica Bianchini Sanchez, Wanderson Geraldo Lima, Gabriel Menezes Yazbeck, Leandro Marcio Moreira, Camila Carrião Machado Garcia

The São Francisco River (SFR), one of the main Brazilian rivers, has suffered cumulative anthropogenic impacts, leading to ever-decreasing fish stocks and environmental, economic, and social consequences. Rhinelepis aspera and Prochilodus argenteus are medium-sized, bottom-feeding, and rheophilic fishes from the SFR that suffer from these actions. Both species are targeted for spawning and restocking operations due to their relevance in artisanal fisheries, commercial activities, and conservation concerns. Using high-throughput sequencing of the 16S rRNA gene, we characterized the microbiome present in the gills and guts of these species recruited from an impacted SFR region and hatchery tanks (HT). Our results showed that bacterial diversity from the gill and gut at the genera level in both fish species from HT is 87% smaller than in species from the SFR. Furthermore, only 15 and 29% of bacterial genera are shared between gills and guts in R. aspera and P. argenteus from SFR, respectively, showing an intimate relationship between functional differences in organs. In both species from SFR, pathogenic, xenobiont-degrading, and cyanotoxin-producer bacterial genera were found, indicating the critical pollution scenario in which the river finds itself. This study allowed us to conclude that the conditions imposed on fish in the HT act as important modulators of microbial diversity in the analyzed tissues. It also raises questions regarding the effects of these conditions on hatchery spawn fish and their suitability for restocking activities, aggravated by the narrow genetic diversity associated with such freshwater systems.

History

Usage metrics

    Frontiers in Microbiology

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC