Table_1_Genome-Wide Identification of DNA Methylases and Demethylases in Kiwifruit (Actinidia chinensis).xlsx (10.77 kB)

Table_1_Genome-Wide Identification of DNA Methylases and Demethylases in Kiwifruit (Actinidia chinensis).xlsx

dataset

posted on 2020-09-09, 04:40 authored by Yaoxin Zhang, Xiaoqing He, Haochen Zhao, Wencai Xu, Heng Deng, Huan Wang, Shuyue Wang, Dan Su, Zhenlei Zheng, Bin Yang, Don Grierson, Jun Wu, Mingchun Liu

DNA methylation plays an important role in a wide range of developmental and physiological processes in plants. It is primarily catalyzed and regulated by cytosine-5 DNA methyltransferases (C5-MTases) and a group of DNA glycosylases that act as demethylases. To date, no genome-scale analysis of the two kiwifruit (Actinidia chinensis) families has been undertaken. In our study, nine C5-MTases and seven DNA demethylase genes were identified in the kiwifruit genome. Through selective evolution analysis, we found that there were gene duplications in C5-MTases and demethylases, which may have arisen during three genome doubling events followed by selection during evolution of kiwifruit. Expression analysis of DNA methylases (C5-MTases) and demethylases identified changes in transcripts of DNA methylation and demethylation genes during both vegetative and reproductive development. Moreover, we found that some members of the two methylase/demethylase families may also be involved in fruit ripening and the regulation of softening. Our results help to better understand the complex roles of methylation/demethylation in plants and provide a foundation for analyzing the role of DNA methylation modification in kiwifruit growth, development and ripening.