Table_1_Functional Characterization of Argininosuccinate Lyase Gene Variants by Mini-Gene Splicing Assay.docx (12.52 kB)

Table_1_Functional Characterization of Argininosuccinate Lyase Gene Variants by Mini-Gene Splicing Assay.docx

Download (12.52 kB)
dataset
posted on 17.05.2019, 12:30 by Yanyun Wang, Yun Sun, Ming Liu, Xiaojuan Zhang, Tao Jiang
Objective

Argininosuccinate lyase (ASL) gene mutations account for argininosuccinic aciduria (ASA). This study aimed to design a minigene construct of ASL gene in order to investigate the impact of variants on splicing.

Methods

The peripheral blood samples were collected from the family members, and genomic DNA was extracted for gene diagnosis using the total exon sequencing method. The novel mutation gene was cloned into pEGFP-C1 vector, and the pathogenicity of the mutation was examined in cultured cells in vitro.

Results

The clinical diagnosis of the proband as ASA was clear. Two pathogenic mutations, c.281G>T (p.Arg94Leu) and c.208-15 T>A were detected in the ASL gene, and the two mutations had not been reported. The minigene expression in vitro confirmed that c.208-15 T>A could cause aberrant splicing, resulting in the retention of 13 bp in intron 2 to exon 3.

Conclusion

Two new pathogenic mutations of ASL gene, c.208-15 T>A and c.281G>T, were found in an ASA family, which enriches the mutational profile of the ASL gene and provides a basis for genetic diagnosis of ASA. Minigenes are optimal approaches to determine whether the intron mutation can cause aberrant splicing.

History

Licence

Exports