Table_1_Expression, Purification, Refolding, and Characterization of a Neverland Protein From Caenorhabditis elegans.DOCX (19.75 kB)

Table_1_Expression, Purification, Refolding, and Characterization of a Neverland Protein From Caenorhabditis elegans.DOCX

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posted on 21.10.2020, 05:00 by Shuhong Mao, Zhan Song, Mian Wu, Xiaorui Wang, Fuping Lu, Hui-Min Qin

Steroid hormones that serve as vital compounds are necessary for the development and metabolism of a variety of organisms. The neverland (NVD) family genes encode the conserved Rieske-type oxygenases, which are accountable for the dehydrogenation during the synthesis and regulation of steroid hormones. However, the His-tagged NVD protein from Caenorhabditis elegans expresses as inclusion bodies in Escherichia coli BL21 (DE3). This bottleneck can be solved through refolding by urea or the introduction of a maltose-binding protein (MBP) tag at the N-terminus. Through further research on purification after the introduction of a MBP tag at the N-terminus, the CD measurement and fluorescence-based thermal shift assay indicated that MBP was favorable for the NVD proteins’ solubility and stability, which may be beneficial for the large-scale manufacture of NVD protein for further research. The structural model contained the Rieske [2Fe–2S] domain and non-heme iron-binding motif, which were similar to 3-ketosteroid 9 α-hydroxylase.

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