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Table_1_Daylight-driven carbon exchange through a vertically structured microbial community.XLSX (17.57 kB)

Table_1_Daylight-driven carbon exchange through a vertically structured microbial community.XLSX

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posted on 2023-05-26, 10:27 authored by James J. Moran, Hans C. Bernstein, Jennifer M. Mobberley, Allison M. Thompson, Young-Mo Kim, Karl L. Dana, Alexandra B. Cory, Steph Courtney, Ryan S. Renslow, James K. Fredrickson, Helen W. Kreuzer, Mary S. Lipton

Interactions between autotrophs and heterotrophs are central to carbon (C) exchange across trophic levels in essentially all ecosystems and metabolite exchange is a frequent mechanism for distributing C within spatially structured ecosystems. Yet, despite the importance of C exchange, the timescales at which fixed C is transferred in microbial communities is poorly understood. We employed a stable isotope tracer combined with spatially resolved isotope analysis to quantify photoautotrophic uptake of bicarbonate and track subsequent exchanges across a vertical depth gradient in a stratified microbial mat over a light-driven diel cycle. We observed that C mobility, both across the vertical strata and between taxa, was highest during periods of active photoautotrophy. Parallel experiments with 13C-labeled organic substrates (acetate and glucose) showed comparably less exchange of C within the mat. Metabolite analysis showed rapid incorporation of 13C into molecules that can both comprise a portion of the extracellular polymeric substances in the system and serve to transport C between photoautotrophs and heterotrophs. Stable isotope proteomic analysis revealed rapid C exchange between cyanobacterial and associated heterotrophic community members during the day with decreased exchange at night. We observed strong diel control on the spatial exchange of freshly fixed C within tightly interacting mat communities suggesting a rapid redistribution, both spatially and taxonomically, primarily during daylight periods.


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