Table_1_A Single-Cell Sequencing Guide for Immunologists.DOCX (1.71 MB)
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Table_1_A Single-Cell Sequencing Guide for Immunologists.DOCX

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posted on 26.02.2019, 09:13 by Peter See, Josephine Lum, Jinmiao Chen, Florent Ginhoux

In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) approaches in the field of immunology. With the wide range of technologies available, it is becoming harder for users to select the best scRNA-seq protocol/platform to address their biological questions of interest. Here, we compared the advantages and limitations of four commonly used scRNA-seq platforms in order to clarify their suitability for different experimental applications. We also address how the datasets generated by different scRNA-seq platforms can be integrated, and how to identify unknown populations of single cells using unbiased bioinformatics methods.