Data_Sheet_5_Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies.ZIP
Herein, we assessed the anti-inflammatory and intestinal barrier protective effects of butyrolactone-I (BTL-1), derived from the coral-derived endophytic fungus (Aspergillus terreus), using the LPS-induced IPEC-J2 inflammation model and the DSS-induced IBD model in mice. In IPEC-J2 cells, pretreatment with BTL-I significantly inhibited TLR4/NF-κB signaling pathway and JNK phosphorylation, resulting in the decrease of IL-1β and IL-6 expression. Interestingly, BTL-1 pretreatment activated the phosphorylation of ERK and P38, which significantly enhanced the expression of TNF-α. Meanwhile, BTL-1 pretreatment upregulated tight junction protein expression (ZO-1, occludin, and claudin-1) and maintained intestinal barrier and intestinal permeability integrity. In mice, BTL-1 significantly alleviated the intestinal inflammatory response induced by DSS, inhibited TLR4/NF-κB signaling pathway, and MAPK signaling pathway, thus reducing the production of IL-1, IL-6, and TNF-α. Further, the expression of tight junction proteins (ZO-1, occludin, and claudin-1) was upregulated in BTL-1 administrated mice. Therefore, it has been suggested that butyrolactone-I alleviates inflammatory responses in LPS-stimulated IPEC-J2 and DSS-induced murine colitis by TLR4/NF-κB and MAPK signal pathway. Thereby, BTL-1 might potentially be used as an ocean drug to prevent intestinal bowel disease.
History
References
- https://doi.org//10.1016/j.intimp.2020.107194
- https://doi.org//10.1038/nrgastro.2013.117
- https://doi.org//10.1111/1751-2980.12290
- https://doi.org//10.1096/fj.202002622R
- https://doi.org//10.1007/s10753-016-0447-7
- https://doi.org//10.1111/jfbc.13244
- https://doi.org//10.2174/1389201021666191216122555
- https://doi.org//10.1039/D0FO00950D
- https://doi.org//10.1016/j.jaci.2009.05.038
- https://doi.org//10.5217/ir.2015.13.1.11
- https://doi.org//10.1136/gutjnl-2014-308419
- https://doi.org//10.1002/JLB.5MR0120-230R
- https://doi.org//10.3390/md16060202
- https://doi.org//10.1016/0378-4347%2893%2980485-M
- https://doi.org//10.1046/j.1471-4159.1998.70041401.x
- https://doi.org//10.1017/S0967199419000571
- https://doi.org//10.1016/j.ejogrb.2009.03.016
- https://doi.org//10.1002/1098-2795%28200010%2957%3A22%3C04%3A%3AAID-MRD123%3E0.CO
- https://doi.org//10.1007/s11274-014-1701-5
- https://doi.org//10.1080/09168451.2020.1764838
- https://doi.org//10.3389/fphar.2019.01354
- https://doi.org//10.3389/fphar.2019.01281
- https://doi.org//10.1038/s41419-019-2157-1
- https://doi.org//10.1016/j.abb.2020.108409
- https://doi.org//10.2337/db07-1403
- https://doi.org//10.1002/jcc.21256
- https://doi.org//10.1002/jcc.21334
- https://doi.org//10.1016/j.jscs.2011.12.002
- https://doi.org//10.1038/s41575-020-00399-w
- https://doi.org//10.1248/cpb.25.2593
- https://doi.org//10.3390/biom10040641
- https://doi.org//10.1016/j.foodres.2019.108756
- https://doi.org//10.2147/JIR.S262132
- https://doi.org//10.1039/C8RA01840E
- https://doi.org//10.1039/C9FO02165E
- https://doi.org//10.1080/15384101.2019.1653106
- https://doi.org//10.1016/j.vetimm.2019.03.012
- https://doi.org//10.1177/2211068214561025
- https://doi.org//10.1039/C6FO01592A
- https://doi.org//10.1152/ajpgi.1995.269.4.G467
- https://doi.org//10.1039/c8fo01123k
- https://doi.org//10.1096/fj.201901061R
- https://doi.org//10.1093/biolre/iox026