Data_Sheet_2_Prediction of Postoperative Ileus in Patients With Colorectal Cancer by Preoperative Gut Microbiota.DOCX (15.91 kB)
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Data_Sheet_2_Prediction of Postoperative Ileus in Patients With Colorectal Cancer by Preoperative Gut Microbiota.DOCX

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posted on 25.11.2020, 04:58 authored by Ye Jin, Rui Geng, Yang Liu, Lujia Liu, Xiangren Jin, Fuya Zhao, Jing Feng, Yunwei Wei
Background

Ileus and postoperative ileus (POI) are common complications of colorectal cancer (CRC). However, little is known about the gut microbiota associated with ileus.

Method

Differences in gut microbiota were evaluated by 16S rRNA gene sequencing. We characterized the gut microbiota in 85 CRC patients (cohort 1) and detected differences, and an independent cohort composed of 38 CRC patients (cohort 2) was used to evaluate the results.

Results

The gut microbiota of CRC patients with and without ileus exhibited large differences in alpha- and beta-diversities and bacterial taxa. The Firmicutes-to-Bacteroidetes ratio and microbial dysbiosis index (MDI) showed greater dysbiosis among ileus patients than among those without ileus. According to the location of CRC, the difference in gut microbiota between patients with and without ileus was more obvious in those with distal CRC than in those with proximal CRC. Finally, Faecalibacterium was significantly reduced in the postoperative perioperative period in patients with ileus. Thus, we used Faecalibacterium as a biomarker for predicting perioperative or POI: the AUC value was 0.74 for perioperative ileus and 0.67 for POI that appeared at 6 months after hospital discharge. The predictive power was evaluated in Cohort 2, with an AUC value of 0.79.

Conclusion

These findings regarding difference of gut microbiota in postoperative CRC patients may provide a theoretical basis for the use of microbiota as biomarkers for the prediction of POI.

History