Data_Sheet_1_Phenotypic and Functional Analysis of Human NK Cell Subpopulations According to the Expression of FcεRIγ and NKG2C.PDF (2.39 MB)
Download file

Data_Sheet_1_Phenotypic and Functional Analysis of Human NK Cell Subpopulations According to the Expression of FcεRIγ and NKG2C.PDF

Download (2.39 MB)
dataset
posted on 2019-12-06, 04:07 authored by Kyung Hwan Kim, Hee Tae Yu, Ilwoong Hwang, Sungha Park, Su-Hyung Park, Sungjin Kim, Eui-Cheol Shin

Human memory-like NK cells are commonly defined by either a lack of FcεRIγ or gain of NKG2C expression. Here, we investigated the heterogeneity of human CD56dim NK cell subpopulations according to the expression of FcεRIγ and NKG2C in a large cohort (n = 127). Although the frequency of FcεRIγ and NKG2C+ NK cells positively correlated, the FcεRIγ and NKG2C+ NK cell populations did not exactly overlap. The FcεRIγ+NKG2C+, FcεRIγNKG2C+, and FcεRIγNKG2C NK cell populations were only evident after HCMV infection, but each had distinct characteristics. Among the subpopulations, FcεRIγNKG2C+ NK cells exhibited the most restricted killer immunoglobulin-like receptor repertoire, suggesting clonal expansion. Moreover, FcεRIγNKG2C+ NK cells exhibited the lowest Ki-67 and highest Bcl-2 expression, indicating the long-lived quiescent memory-like property. Functionally, FcεRIγNKG2C+ NK cells had weak natural effector function against K562 but strong effector functions by CD16 engagement, whereas FcεRIγ+NKG2C+ NK cells had strong effector functions in both settings. Anatomically, the FcεRIγ+NKG2C+, FcεRIγNKG2C+, and FcεRIγNKG2C NK cell populations were present in multiple human peripheral organs. In conclusion, we demonstrate the heterogeneity of memory-like NK cells stratified by FcεRIγ and NKG2C and suggest both markers be utilized to better define these cells.

History