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Data_Sheet_1_Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.docx (2.08 MB)
Data_Sheet_1_Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.docx
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posted on 02.05.2019, 15:20 authored by Lauren Gwynne, Adam C. Sedgwick, Jordan E. Gardiner, George T. Williams, Gyoungmi Kim, John P. Lowe, Jean-Yves Maillard, A. Toby A. Jenkins, Steven D. Bull, Jonathan L. Sessler, Juyoung Yoon, Tony D. JamesA long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
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References
- https://doi.org//10.1016/S0006-291X(86)80456-9
- https://doi.org//10.1021/ol702539v
- https://doi.org//10.1136/mp.55.2.65
- https://doi.org//10.1039/C6TB00828C
- https://doi.org//10.1146/annurev.bb.21.060192.002301
- https://doi.org//10.1002/bio.2977
- https://doi.org//10.1021/ja039768k
- https://doi.org//10.1038/cddis.2014.194
- https://doi.org//10.3389/fchem.2018.00618
- https://doi.org//10.1016/j.aca.2016.11.012
- https://doi.org//10.1021/ac300983t
- https://doi.org//10.1007/s00216-010-4060-6
- https://doi.org//10.1038/nrc2980
- https://doi.org//10.1074/jbc.M404145200
- https://doi.org//10.1111/j.1432-1033.1984.tb07879.x
- https://doi.org//10.1039/C4NJ00935E
- https://doi.org//10.1021/ja057903i
- https://doi.org//10.1016/j.talanta.2017.04.081
- https://doi.org//10.1021/ja802883k
- https://doi.org//10.1146/annurev.med.60.042307.110714
- https://doi.org//10.1002/3527608060
- https://doi.org//10.1126/science.8248785
- https://doi.org//10.1002/jcla.20178
- https://doi.org//10.1016/j.sjbs.2015.03.013
- https://doi.org//10.1021/ac504519b
- https://doi.org//10.1111/j.1600-079X.2006.00318.x
- https://doi.org//10.1002/eat.22436
- https://doi.org//10.1021/ac0522106
- https://doi.org//10.15406/jcpcr.2018.09.00320
- https://doi.org//10.1039/C7CC05872A
- https://doi.org//10.1039/C8CC01661E
- https://doi.org//10.1039/C7CC07845E
- https://doi.org//10.1039/C8SC00733K
- https://doi.org//10.1021/ac501814u
- https://doi.org//10.1016/0006-291X(82)90635-0
- https://doi.org//10.1021/acsami.6b14176
- https://doi.org//10.1016/j.tetlet.2018.06.016
- https://doi.org//10.1016/0009-8981(88)90136-2
- https://doi.org//10.3892/mmr.2015.3524
- https://doi.org//10.1039/C7CS00240H
- https://doi.org//10.1046/j.1464-410x.2001.02275.x
- https://doi.org//10.1016/j.bios.2015.10.003
- https://doi.org//10.1016/j.biomaterials.2017.06.032
- https://doi.org//10.1039/C5CC01005E
- https://doi.org//10.1039/C7CS00907K
- https://doi.org//10.1039/C5AN00516G
- https://doi.org//10.1016/j.bios.2016.12.025
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Categories
- Geochemistry
- Biochemistry
- Organic Chemistry
- Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
- Nuclear Chemistry
- Medical Biochemistry and Metabolomics not elsewhere classified
- Analytical Biochemistry
- Cell Neurochemistry
- Physical Organic Chemistry
- Enzymes
- Organic Green Chemistry
- Environmental Chemistry (incl. Atmospheric Chemistry)
- Catalysis and Mechanisms of Reactions
- Electroanalytical Chemistry
- Analytical Chemistry not elsewhere classified
- Environmental Chemistry
- Food Chemistry and Molecular Gastronomy (excl. Wine)
- Inorganic Chemistry