Data_Sheet_1_Integrative Epigenomic Analysis of Transcriptional Regulation of Human CircRNAs.docx (2.29 MB)
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Data_Sheet_1_Integrative Epigenomic Analysis of Transcriptional Regulation of Human CircRNAs.docx

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posted on 25.01.2021, 04:56 authored by Xue-Cang Li, Zhi-Dong Tang, Li Peng, Yan-Yu Li, Feng-Cui Qian, Jian-Mei Zhao, Ling-Wen Ding, Xiao-Juan Du, Meng Li, Jian Zhang, Xue-Feng Bai, Jiang Zhu, Chen-Chen Feng, Qiu-Yu Wang, Jian Pan, Chun-Quan Li

Circular RNAs (circRNAs) are evolutionarily conserved and abundant non-coding RNAs whose functions and regulatory mechanisms remain largely unknown. Here, we identify and characterize an epigenomically distinct group of circRNAs (TAH-circRNAs), which are transcribed to a higher level than their host genes. By integrative analysis of cistromic and transcriptomic data, we find that compared with other circRNAs, TAH-circRNAs are expressed more abundantly and have more transcription factors (TFs) binding sites and lower DNA methylation levels. Concordantly, TAH-circRNAs are enriched in open and active chromatin regions. Importantly, ChIA-PET results showed that 23–52% of transcription start sites (TSSs) of TAH-circRNAs have direct interactions with cis-regulatory regions, strongly suggesting their independent transcriptional regulation from host genes. In addition, we characterize molecular features of super-enhancer-driven circRNAs in cancer biology. Together, this study comprehensively analyzes epigenomic characteristics of circRNAs and identifies a distinct group of TAH-circRNAs that are independently transcribed via enhancers and super-enhancers by TFs. These findings substantially advance our understanding of the regulatory mechanism of circRNAs and may have important implications for future investigations of this class of non-coding RNAs.

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