Data_Sheet_1_Exploring Specific miRNA-mRNA Axes With Relationship to Taxanes-Resistance in Breast Cancer.doc (29.5 kB)

Data_Sheet_1_Exploring Specific miRNA-mRNA Axes With Relationship to Taxanes-Resistance in Breast Cancer.doc

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posted on 21.08.2020, 15:14 by Danni Chen, Chang Bao, Feng Zhao, Haogang Yu, Guansheng Zhong, Liang Xu, Senxiang Yan

Breast cancer is the most prevalent type of malignancy in women worldwide. Taxanes (paclitaxel and docetaxel) are widely applied as first-line chemotherapeutic agents, while the therapeutic effect is seriously limited by the development of drug resistance. In the present study, we screened out several miRNAs dysregulated in taxanes-resistant breast cancer samples and confirmed that two miRNAs (miR-335-5p and let-7c-5p) played a major role in cell proliferation, apoptosis, and chemo-resistance. In addition, the weighted gene co-expression network analysis (WGCNA) for potential target genes of miR-335-5p and let-7c-5p identified three hub genes (CXCL9, CCR7, and SOCS1) with a positive relationship to taxanes-sensitivity. Further, target relationships between miR-335-5p and CXCL9, let-7c-5p and CCR7/SOCS1 were confirmed by dual-luciferase reporter assays. Importantly, the regulatory functions of CXCL9, CCR7, and SOCS1 on proliferation and chemoresistance were validated. In conclusion, our study shed light on clinical theragnostic relationships between miR-335-5p/CXCL9, let-7c-5p/CCR7/SOCS1 axes, and taxanes-resistance in breast cancer.

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