Data_Sheet_1_Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly.PDF (557.69 kB)

Data_Sheet_1_Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly.PDF

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posted on 01.02.2019 by Hiroki Sakuta, Tadashi Fujimoto, Yusuke Yamana, Yusuke Hoda, Kanta Tsumoto, Kenichi Yoshikawa

To artificially construct a three-dimensional cell assembly, we investigated the availability of long-duration microdroplets that emerged near a critical point in an aqueous two-phase system (ATPS) with the hydrophilic binary polymers, polyethylene glycol (PEG), and dextran (DEX), as host containers. We found that erythrocytes (horse red blood cells; RBCs) and NAMRU mouse mammary gland epithelial cells (NMuMG cells) were completely and spontaneously entrapped inside DEX-rich microdroplets. RBCs and NMuMG cells were located in the interior and at the periphery of the droplets at PEG/DEX = 5%:5%. In contrast, the cells exhibited opposite localizations at PEG/DEX = 10%:5%, where, interestingly, NMuMG cells apparently assembled to achieve cell adhesion. We simply interpreted such specific localizations by considering the alternative responses of these cells to the properties of the PEG/DEX interfaces with different gradients in polymer concentrations.

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