Data_Sheet_1_Altered Immunity and Microbial Dysbiosis in Aged Individuals With Long-Term Controlled HIV Infection.PDF (7.92 MB)

Data_Sheet_1_Altered Immunity and Microbial Dysbiosis in Aged Individuals With Long-Term Controlled HIV Infection.PDF

Download (7.92 MB)
dataset
posted on 12.03.2019, 04:52 by Nicholas Rhoades, Norma Mendoza, Allen Jankeel, Suhas Sureshchandra, Alexander D. Alvarez, Brianna Doratt, Omeid Heidari, Rod Hagan, Brandon Brown, Steven Scheibel, Theodore Marbley, Jeff Taylor, Ilhem Messaoudi

The introduction of highly active antiretroviral therapy (HAART) resulted in a significant increase in life expectancy for HIV patients. Indeed, in 2015, 45% of the HIV+ individuals in the United States were ≥55 years of age. Despite improvements in diagnosis and treatment of HIV infection, geriatric HIV+ patients suffer from higher incidence of comorbidities compared to age-matched HIV- individuals. Both chronic inflammation and dysbiosis of the gut microbiome are believed to be major contributors to this phenomenon, however carefully controlled studies investigating the impact of long-term (>10 years) controlled HIV (LTC-HIV) infection are lacking. To address this question, we profiled circulating immune cells, immune mediators, and the gut microbiome from elderly (≥55 years old) LTC-HIV+ and HIV- gay men living in the Palm Springs area. LTC-HIV+ individuals had lower frequency of circulating monocytes and CD4+ T-cells, and increased frequency CD8+ T-cells. Moreover, levels of systemic INFγ and several growth factors were increased while levels of IL-2 and several chemokines were reduced. Upon stimulation, immune cells from LTC-HIV+ individuals produced higher levels of pro-inflammatory cytokines. Last but not least, the gut microbiome of LTC-HIV+ individuals was enriched in bacterial taxa typically found in the oral cavity suggestive of loss of compartmentalization, while levels of beneficial butyrate producing taxa were reduced. Additionally, prevalence of Prevotella negatively correlated with CD4+ T-cells numbers in LTC-HIV+ individuals. These results indicate that despite long-term adherence and undetectable viral loads, LTC-HIV infection results in significant shifts in immune cell frequencies and gut microbial communities.

History

References

Licence

Exports