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DataSheet_1_The deficiency in Th2-like Tfh cells affects the maturation and quality of HIV-specific B cell response in viremic infection.pdf (23.05 MB)

DataSheet_1_The deficiency in Th2-like Tfh cells affects the maturation and quality of HIV-specific B cell response in viremic infection.pdf

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posted on 2022-08-24, 10:55 authored by Alessandra Noto, Madeleine Suffiotti, Victor Joo, Antonio Mancarella, Francesco A. Procopio, Guy Cavet, Yvonne Leung, Jean-Marc Corpataux, Matthias Cavassini, Agostino Riva, Leonidas Stamatatos, Raphael Gottardo, Adrian B. McDermott, Richard A. Koup, Craig Fenwick, Matthieu Perreau, Giuseppe Pantaleo

Optimal T follicular helper (Tfh) cells function is important to promote the development of germinal centers and maturation of high affinity antigen-specific B cells. We have found that the expression of CXCR3 defines distinct Tfh subsets: CXCR3+ Th1-like Tfh cells mainly producing single IFN-γ and dual IL-21/IFN-γ and CXCR3- Th2-like Tfh cells mainly producing single IL-4 and dual IL-21/IL-4 cytokines. CXCR3- Th2-like Tfhs are significantly reduced during ongoing HIV replication. While the percentage of Th2-like Tfh cells correlates with that of total and cycling HIV-specific B cells, the percentage of CXCR3+ Th1-like Tfhs correlates with HIV-specific B cells expressing T-bet and CXCR3. Of note, only IL-4 and IL-21 cytokines boosted efficient maturation of HIV-specific B cells while IFN-γ induced expression of T-bet and CXCR3 in B cells. Interestingly, total and HIV-specific CXCR3+ B cells showed lower rate of somatic hypermutation, as compared to CXCR3- B cells. Therefore, the imbalance in Th2/Th1-like Tfhs affects B cell responses in viremic HIV infection.

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