DataSheet4_Identification of Non-Canonical Translation Products in C. elegans Using Tandem Mass Spectrometry.fasta (22.23 MB)
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DataSheet4_Identification of Non-Canonical Translation Products in C. elegans Using Tandem Mass Spectrometry.fasta

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posted on 25.10.2021, 16:42 authored by Bhavesh S. Parmar, Marlies K. R. Peeters, Kurt Boonen, Ellie C. Clark, Geert Baggerman, Gerben Menschaert, Liesbet Temmerman

Transcriptome and ribosome sequencing have revealed the existence of many non-canonical transcripts, mainly containing splice variants, ncRNA, sORFs and altORFs. However, identification and characterization of products that may be translated out of these remains a challenge. Addressing this, we here report on 552 non-canonical proteins and splice variants in the model organism C. elegans using tandem mass spectrometry. Aided by sequencing-based prediction, we generated a custom proteome database tailored to search for non-canonical translation products of C. elegans. Using this database, we mined available mass spectrometric resources of C. elegans, from which 51 novel, non-canonical proteins could be identified. Furthermore, we utilized diverse proteomic and peptidomic strategies to detect 40 novel non-canonical proteins in C. elegans by LC-TIMS-MS/MS, of which 6 were common with our meta-analysis of existing resources. Together, this permits us to provide a resource with detailed annotation of 467 splice variants and 85 novel proteins mapped onto UTRs, non-coding regions and alternative open reading frames of the C. elegans genome.

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