DataSheet1_Roles of Krüppel Homolog 1 and Broad-Complex in the Development of Dendroctonus armandi (Coleoptera: Scolytinae).docx
In insects, metamorphosis is controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E). Krüppel homolog 1 (Kr-h1), a key JH-early inducible gene, is responsible for the suppression of metamorphosis and the regulation of the Broad-Complex (Br-C) gene, which is induced by 20E and functions as a “pupal specifier”. In this study, we identified and characterized the expression patterns and tissue distribution of DaKr-h1 and DaBr-C at various developmental stages of Dendroctonus armandi. The expression of the two genes was induced by JH analog (JHA) methoprene and 20E, and their functions were investigated by RNA interference. DaKr-h1 and DaBr-C were predominantly expressed in the heads of larvae and were significantly downregulated during the molting stage. In contrast, the DaKr-h1 transcript level was highest in the adult anterior midgut. DaBr-C was mainly expressed in female adults, with the highest transcript levels in the ovaries. In the larval and pupal stages, both JHA and 20E significantly induced DaKr-h1, but only 20E significantly induced DaBr-C, indicating the importance of hormones in metamorphosis. DaKr-h1 knockdown in larvae upregulated DaBr-C expression, resulting in precocious metamorphosis from larvae to pupae and the formation of miniature pupae. DaKr-h1 knockdown in pupae suppressed DaBr-C expression, increased emergence, caused abnormal morphology, and caused the formation of small-winged adults. These results suggest that DaKr-h1 is required for the metamorphosis of D. armandi. Our findings provide insight into the roles of DaKr-h1 and DaBr-C in JH-induced transcriptional repression and highlight DaKr-h1 as a potential target for metamorphosis suppression in D. armandi.
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References
- https://doi.org//10.1016/j.ibmb.2011.09.003
- https://doi.org//10.1006/jmbi.1990.999910.1016/s0022-2836(05)80360-2
- https://doi.org//10.1006/dbio.1996.0140
- https://doi.org//10.1006/dbio.1997.8620
- https://doi.org//10.1016/j.cbpb.2013.02.004
- https://doi.org//10.1677/jme.1.01531
- https://doi.org//10.1016/j.ibmb.2015.08.004
- https://doi.org//10.1111/imb.12108
- https://doi.org//10.1073/pnas.1506645112
- https://doi.org//10.1242/dev.124.22.4639
- https://doi.org//10.1093/genetics/129.2.385
- https://doi.org//10.1016/j.jinsphys.2020.104041
- https://doi.org//10.1016/j.ygcen.2012.01.005
- https://doi.org//10.1006/jmbi.2000.3903
- https://doi.org//10.3389/fphys.2019.00967
- https://doi.org//10.1111/eea.12991
- https://doi.org//10.1111/jen.12941
- https://doi.org//10.1385/1-59259-890-0%3A571
- https://doi.org//10.1109/SMElec.2012.6417228
- https://doi.org//10.1007/s00359-006-0202-x
- https://doi.org//10.1073/pnas.2335884100
- https://doi.org//10.1016/j.bbagen.2012.09.025
- https://doi.org//10.1111/1744-7917.12411
- https://doi.org//10.1016/S2095-3119(13)60654-1
- https://doi.org//10.1046/j.1365-2443.1997.1460344.x
- https://doi.org//10.1073/pnas.1615423114
- https://doi.org//10.1073/pnas.1204951109
- https://doi.org//10.1016/j.ydbio.2014.01.022
- https://doi.org//10.1074/jbc.M115.686121
- https://doi.org//10.1007/s13355-015-0345-8
- https://doi.org//10.1038/nrg1581
- https://doi.org//10.1093/genetics/118.2.247
- https://doi.org//10.1038/262136a0
- https://doi.org//10.1242/dev.016097
- https://doi.org//10.1371/journal.pone.0028728
- https://doi.org//10.1093/molbev/msw054
- https://doi.org//10.1006/dbio.1999.9343
- https://doi.org//10.1093/molbev/msn067
- https://doi.org//10.3389/fphys.2018.00291
- https://doi.org//10.3389/fphys.2021.662651
- https://doi.org//10.1073/pnas.1716897115
- https://doi.org//10.1038/srep00163
- https://doi.org//10.1016/j.ydbio.2008.10.016
- https://doi.org//10.1111/j.1742-4658.2008.06428.x
- https://doi.org//10.1016/j.ibmb.2010.11.004
- https://doi.org//10.1016/j.mod.2007.10.002
- https://doi.org//10.1016/j.mod.2008.02.001
- https://doi.org//10.1016/j.mod.2007.11.001
- https://doi.org//10.1242/dev.102020
- https://doi.org//10.1006/dbio.2000.9687
- https://doi.org//10.1016/j.cbpc.2004.09.009
- https://doi.org//10.1002/(SICI)1520-6327(1996)32%3A3/4<271%3AAID-ARCH2>3.0.CO;2-W
- https://doi.org//10.1016/S0065-2806(08)60084-3
- https://doi.org//10.1016/s0083-6729(00)60016-x
- https://doi.org//10.1016/j.ygcen.2012.06.001
- https://doi.org//10.1242/dev.037218
- https://doi.org//10.1002/dneu.20537
- https://doi.org//10.1016/j.ydbio.2014.03.006
- https://doi.org//10.1002/arch.21828
- https://doi.org//10.3390/ijms23052749.c
- https://doi.org//10.1242/dev.015263
- https://doi.org//10.1038/sj.cdd.4401587
- https://doi.org//10.1371/journal.pgen.1006020
- https://doi.org//10.1073/pnas.1401478111
- https://doi.org//10.1016/j.mod.2004.10.006
- https://doi.org//10.1126/science.276.5309.114
- https://doi.org//10.1016/j.bbagen.2014.05.013
- https://doi.org//10.1038/srep23136
- https://doi.org//10.1111/imb.12389
- https://doi.org//10.1074/jbc.M110.191684
- https://doi.org//10.1007/s00049-017-0235-3
- https://doi.org//10.1006/dbio.1998.9059
- https://doi.org//10.1006/dbio.2000.0143
- https://doi.org//10.1242/dev.129.9.2259
- https://doi.org//10.1016/j.ibmb.2009.12.004