DataSheet1_Biotechnologically Produced Lavandula angustifolia Mill. Extract Rich in Rosmarinic Acid Resolves Psoriasis-Related Inflammation Through Janus Kinase/Signal Transducer and Activator of Transcription Signaling.docx
Psoriasis is a common skin pathology, characterized by dysregulation of epidermal keratinocyte function attended by persistent inflammation, suggesting that molecules with anti-inflammatory potential may be effective for its management. Rosmarinic acid (RA) is a natural bioactive molecule known to have an anti-inflammatory potential. Here we examined the effect of biotechnologically produced cell suspension extract of Lavandula angustifolia Mill (LV) high in RA content as treatment for psoriasis-associated inflammation in human keratinocytes. Regulatory genes from the nuclear factor kappa B (NF-κB) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathways were upregulated upon stimulation with a combination of interferon gamma (IFN-γ), interleukin (IL)-17A and IL-22. We also observed that both LV extract and RA could inhibit JAK2, leading to reduced STAT1 phosphorylation. Further, we demonstrated that LV extract inhibited phosphoinositide 3-kinases (PI3K) and protein kinase B (AKT), which could be implicated in reduced hyperproliferation in keratinocytes. Collectively, these findings indicate that the biotechnologically produced LV extract resolved psoriasis-like inflammation in human keratinocytes by interfering the JAK2/STAT1 signaling pathway and its effectiveness is due to its high content of RA (10%). Hence, both LV extract and pure RA possess the potential to be incorporated in formulations for topical application as therapeutic approach against psoriasis.
History
References
- https://doi.org//10.3389/fimmu.2018.01549
- https://doi.org//10.1111/bph.14487
- https://doi.org//10.1038/jid.2013.182
- https://doi.org//10.1128/MCB.05300-11
- https://doi.org//10.1038/s41573-020-00114-z
- https://doi.org//10.1038/s41419-018-0673-z
- https://doi.org//10.1016/j.indcrop.2018.10.081
- https://doi.org//10.1111/jcmm.13322
- https://doi.org//10.3390/antiox8030076
- https://doi.org//10.1016/j.fct.2014.02.002
- https://doi.org//10.1038/nrg1879
- https://doi.org//10.4049/jimmunol.169.1.434
- https://doi.org//10.1016/j.procbio.2006.05.007
- https://doi.org//10.1016/j.jep.2012.10.035
- https://doi.org//10.1007/s11240-015-0840-1
- https://doi.org//10.1016/j.biotechadv.2012.09.006
- https://doi.org//10.1038/nrdp.2016.82
- https://doi.org//10.1111/bjd.12049
- https://doi.org//10.1016/j.jid.2018.09.037
- https://doi.org//10.1016/j.intimp.2011.04.007
- https://doi.org//10.3390/ijms20082027
- https://doi.org//10.1038/jid.2013.453
- https://doi.org//10.3390/nu10101399
- https://doi.org//10.1007/s00217-008-0842-x
- https://doi.org//10.1016/j.jaci.2018.05.036
- https://doi.org//10.3389/fphar.2019.00573
- https://doi.org//10.1016/j.jid.2018.06.184
- https://doi.org//10.1038/s41598-020-60050-1
- https://doi.org//10.1016/j.intimp.2016.10.010
- https://doi.org//10.1016/j.jid.2018.07.032
- https://doi.org//10.1038/nature05663
- https://doi.org//10.3389/fphar.2020.00153
- https://doi.org//10.1016/j.phytol.2016.12.030
- https://doi.org//10.3390/molecules25082006
- https://doi.org//10.1080/07388551.2020.1731414
- https://doi.org//10.3390/nu12051282
- https://doi.org//10.3390/nu12051282
- https://doi.org//10.1111/j.1365-2133.2008.08769.x
- https://doi.org//10.1080/10408398.2015.1006768
- https://doi.org//10.1016/j.foodres.2013.09.016
- https://doi.org//10.1007/s11101-013-9282-8
- https://doi.org//10.1016/j.jep.2020.113127
- https://doi.org//10.1016/j.jaci.2019.06.019
- https://doi.org//10.1016/j.fct.2008.11.026
- https://doi.org//10.1038/nrd.2017.201
- https://doi.org//10.1080/1028415X.2015.1103460
- https://doi.org//10.1111/bph.13631
- https://doi.org//10.1038/jid.2011.222
- https://doi.org//10.1016/j.jaut.2017.11.002
- https://doi.org//10.1038/s41419-019-1510-8
- https://doi.org//10.1038/s41419-019-2212-y
- https://doi.org//10.1016/j.fct.2021.112002
- https://doi.org//10.1126/scitranslmed.3001108
- https://doi.org//10.1002/eji.200535503
- https://doi.org//10.1021/jf8016833
- https://doi.org//10.1016/j.jid.2017.09.005
- https://doi.org//10.1016/j.procbio.2020.11.026
CC BY 4.0