Table_5_Selenium-Enriched Pollen Grains of Olea europaea L.: Ca2+ Signaling and Germination Under Oxidative Stress.xlsx

Selenium (Se) shows antioxidant properties that can be exploited in plants to combat abiotic stresses caused by reactive oxygen species produced in excess (ROS). Here, we show that the Se-fertilization of olive trees with sodium selenate effectively protects the pollen from oxidative stress. Pollen isolated from plants treated with Se or from untreated controls was incubated in vitro with H₂O₂ to produce an oxidative challenge. Given the impact of ROS on Ca²⁺ homeostasis and Ca²⁺-dependent signaling, cytosolic Ca²⁺ was measured to monitor cellular perturbations. We found that H₂O₂ interrupted Ca²⁺ homeostasis only in untreated pollen, while in samples treated in vitro with sodium selenate or selenium methionine, Ca²⁺ homeostasis was preserved. Furthermore, germination rates were considerably better maintained in Se-fertilized pollen compared to non-fertilized pollen (30% vs. 15%, respectively) after exposure to 1 mM H₂O₂. The same was observed with pollen treated in vitro with Se-methionine, which is the organic form of Se, in which part of the fertigated sodium selenate is converted in the plant. Combined, our results show a close correlation between ROS, Ca²⁺ homeostasis, and pollen fertility and provide clear evidence that Se-fertilization is a potential approach to preserve or improve agricultural productivity.