Presentation_1_Spring Is Coming: Genetic Analyses of the Bud Break Date Locus Reveal Candidate Genes From the Cold Perception Pathway to Dormancy Release in Apple (Malus × domestica Borkh.).zip

Miotto, Yohanna Evelyn; Tessele, Carolina; Czermainski, Ana Beatriz Costa; Porto, Diogo Denardi; da Silveira Falavigna, Vítor; Sartor, Tiago; Cattani, Amanda Malvessi; Delatorre, Carla Andrea; Amorim de Alencar, Sérgio; da Silva-Junior, Orzenil Bonfim; Coiti Togawa, Roberto; Costa, Marcos Mota do Carmo; Pappas, Georgios Joannis; Grynberg, Priscila; de Oliveira, Paulo Ricardo Dias; Kvitschal, Marcus Vinícius; Denardi, Frederico; Buffon, Vanessa; Revers, Luís Fernando

doi:10.3389/fpls.2019.00033.s001

Presentation_1_Spring Is Coming: Genetic Analyses of the Bud Break Date Locus Reveal Candidate Genes From the Cold Perception Pathway to Dormancy Rele.zip (3.31 MB)

Presentation_1_Spring Is Coming: Genetic Analyses of the Bud Break Date Locus Reveal Candidate Genes From the Cold Perception Pathway to Dormancy Release in Apple (Malus × domestica Borkh.).zip

presentation

posted on 2019-03-07, 04:24 authored by Yohanna Evelyn Miotto, Carolina Tessele, Ana Beatriz Costa Czermainski, Diogo Denardi Porto, Vítor da Silveira Falavigna, Tiago Sartor, Amanda Malvessi Cattani, Carla Andrea Delatorre, Sérgio Amorim de Alencar, Orzenil Bonfim da Silva-Junior, Roberto Coiti Togawa, Marcos Mota do Carmo Costa, Georgios Joannis Pappas, Priscila Grynberg, Paulo Ricardo Dias de Oliveira, Marcus Vinícius Kvitschal, Frederico Denardi, Vanessa Buffon, Luís Fernando Revers

Chilling requirement (CR) for bud dormancy completion determines the time of bud break in apple (Malus × domestica Borkh.). The molecular control of bud dormancy is highly heritable, suggesting a strong genetic control of the trait. An available Infinium II SNP platform for genotyping containing 8,788 single nucleotide polymorphic markers was employed, and linkage maps were constructed in a F₁ cross from the low CR M13/91 and the moderate CR cv. Fred Hough. These maps were used to identify quantitative trait loci (QTL) for bud break date as a trait related to dormancy release. A major QTL for bud break was detected at the beginning of linkage group 9 (LG9). This QTL remained stable during seven seasons in two different growing sites. To increase mapping efficiency in detecting contributing genes underlying this QTL, 182 additional SNP markers located at the locus for bud break were used. Combining linkage mapping and structural characterization of the region, the high proportion of the phenotypic variance in the trait explained by the QTL is related to the coincident positioning of Arabidopsis orthologs for ICE1, FLC, and PRE1 protein-coding genes. The proximity of these genes from the most explanatory markers of this QTL for bud break suggests potential genetic additive effects, reinforcing the hypothesis of inter-dependent mechanisms controlling dormancy induction and release in apple trees.