Table_1_Peptide Occurring in Enterobacteriaceae Triggers Streptococcus pneumoniae Cell Death.XLSX Fauzy Nasher Min Jung Kwun Nicholas J. Croucher Manfred Heller Lucy J. Hathaway 10.3389/fcimb.2019.00320.s002 https://frontiersin.figshare.com/articles/dataset/Table_1_Peptide_Occurring_in_Enterobacteriaceae_Triggers_Streptococcus_pneumoniae_Cell_Death_XLSX/9792530 <p>Non-encapsulated Streptococcus pneumoniae often possess two genes, aliB-like ORF 1 and aliB-like ORF 2, in place of capsule genes. AliB-like ORF 1 is thought to encode a substrate binding protein of an ABC transporter which binds peptide SETTFGRDFN, found in 50S ribosomal subunit protein L4 of Enterobacteriaceae. Here, we investigated the effect of binding of AliB-like ORF 1 peptide on the transcriptome and proteome of non-encapsulated pneumococci. We found upregulation of gene expression of a metacaspase and a gene encoding N-acetylmuramoyl-L-alanine amidase, both of which are proposed to be involved in programmed cell death in prokaryotic cells. Proteome profiling indicated upregulation of transcriptional regulators and downregulation of metabolism-associated genes. Exposure to the peptide specifically triggered death in pneumococci which express AliB-like ORF 1, with the bacteria having an apoptotic appearance by electron microscopy. We propose that binding of the AliB-like ORF 1 peptide ligand by the pneumococcus signals a challenging environment with hostile bacterial species leading to death of a proportion of the pneumococcal population.</p> 2019-09-10 13:52:50 Streptococcus pneumoniae peptide non-encapsulated aliB-like ORF 1 transcriptome proteome cell death