Lemmens, Liesbeth Tilleman, Laurentijn De Koning, Ezra Valegård, Karin Lindås, Ann-Christin Van Nieuwerburgh, Filip Maes, Dominique Peeters, Eveline Data_Sheet_1_YtrASa, a GntR-Family Transcription Factor, Represses Two Genetic Loci Encoding Membrane Proteins in Sulfolobus acidocaldarius.PDF <p>In bacteria, the GntR family is a widespread family of transcription factors responsible for the regulation of a myriad of biological processes. In contrast, despite their occurrence in archaea only a little information is available on the function of GntR-like transcription factors in this domain of life. The thermoacidophilic crenarchaeon Sulfolobus acidocaldarius harbors a GntR-like regulator belonging to the YtrA subfamily, encoded as the first gene in an operon with a second gene encoding a putative membrane protein. Here, we present a detailed characterization of this regulator, named YtrA<sub>Sa</sub>, with a focus on regulon determination and mechanistic analysis with regards to DNA binding. Genome-wide chromatin immunoprecipitation and transcriptome experiments, the latter employing a ytrA<sub>Sa</sub> overexpression strain, demonstrate that the regulator acts as a repressor on a very restricted regulon, consisting of only two targets including the operon encoding its own gene and a distinct genetic locus encoding another putative membrane protein. For both targets, a conserved 14-bp semi-palindromic binding motif was delineated that covers the transcriptional start site and that is surrounded by additional half-site motifs. The crystallographic structure of YtrA<sub>Sa</sub> was determined, revealing a compact dimeric structure in which the DNA-binding motifs are oriented ideally to enable a specific high-affinity interaction with the core binding motif. This study provides new insights into the functioning of a YtrA-like regulator in the archaeal domain of life.</p> archaea;Sulfolobus;transcription regulation;GntR;YtrA;membrane protein 2019-09-10
    https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_YtrASa_a_GntR-Family_Transcription_Factor_Represses_Two_Genetic_Loci_Encoding_Membrane_Proteins_in_Sulfolobus_acidocaldarius_PDF/9789815
10.3389/fmicb.2019.02084.s001