%0 Generic %A DeWolfe, David %A Aid, Malika %A McGann, Kevin %A Ghofrani, Joshua %A Geiger, Emma %A Helzer, Catherine %A Malik, Shaily %A Kleiboeker, Steve %A Jost, Stephanie %A Tan, Chen Sabrina %D 2019 %T Data_Sheet_3_NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates.docx %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_3_NK_Cells_Contribute_to_the_Immune_Risk_Profile_in_Kidney_Transplant_Candidates_docx/9724865 %R 10.3389/fimmu.2019.01890.s003 %2 https://frontiersin.figshare.com/ndownloader/files/17417492 %K NK cells %K immune risk profile %K BK virus %K kidney transplant %K immune senescence %K end stage renal disease %K dialysis %X

Background: A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with specific NK cell characteristics and ImmuKnow® value.

Material and Methods: Sixty five subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP.

Results: We identified 14 IRP+ [CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (28CD–/CD57+) T cells] individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP– and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition. ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features.

Conclusion: NK cells contribute to overall immune senescence in kidney transplant candidates.

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